{"id":"4611ee3b-522a-5916-9760-f2ca33e3bba9","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-arginine-stress-hormones","predicate":"with_arginine_enhanced_stress_evoked","statement":"In 29 high-trait-anxiety participants, lysine plus arginine increased stress-evoked ACTH, cortisol, adrenaline, and noradrenaline responses without changing heart-rate or blood-pressure responses.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f0ec8be4-e0d1-5a60-9df4-c453a1e70cff","mechanism_event_label":"The mixture did not simply suppress all stress hormones.","subject":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"object":{"id":"f7c11abc-a8ac-515a-a4ca-f7679cc9a472","slug":"neuroendocrine-stress-response","display_name":"Neuroendocrine stress response","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f0ec8be4-e0d1-5a60-9df4-c453a1e70cff","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-arginine-stress-hormones-event","event_type":"observed_intervention","label":"The mixture did not simply suppress all stress hormones.","description":"In 29 high-trait-anxiety participants, lysine plus arginine increased stress-evoked ACTH, cortisol, adrenaline, and noradrenaline responses without changing heart-rate or blood-pressure responses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f7c11abc-a8ac-515a-a4ca-f7679cc9a472","slug":"neuroendocrine-stress-response","display_name":"Neuroendocrine stress response","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"cointervention","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"daf3e4cb-64bf-5ec6-a046-c0a1bedfb477","slug":"acth","display_name":"Adrenocorticotropic hormone","entity_type_key":"protein"},"role":"increased_stress_evoked_response","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b206e39e-112c-558b-91b6-16504d0c518e","slug":"cortisol","display_name":"Cortisol","entity_type_key":"small_molecule"},"role":"increased_stress_evoked_response","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7f5bdfe9-54d7-5cfc-86d9-10c23bdfa290","slug":"epinephrine","display_name":"Epinephrine","entity_type_key":"small_molecule"},"role":"increased_stress_evoked_response","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"34ab29ee-eb2b-5e51-9d25-0c1f8d5b97d9","slug":"norepinephrine","display_name":"Norepinephrine","entity_type_key":"small_molecule"},"role":"increased_stress_evoked_response","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"c86c7e33-27e0-5e40-86d8-2cba0d36172b","slug":"heart-rate-response-to-stress","display_name":"Heart-rate response to stress","entity_type_key":"cellular_process"},"role":"unchanged_response","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""},{"entity":{"id":"918c4501-ee44-523e-8ecc-9e09b894cacb","slug":"blood-pressure-response-to-stress","display_name":"Blood-pressure response to stress","entity_type_key":"cellular_process"},"role":"unchanged_response","stoichiometry":null,"state_label":"","sequence_order":8,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Ten-day randomized trial; 3 g/day of each amino acid, followed by public-speaking stress","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small combination trial; authors' proposed normalization is an interpretation, not a proven lysine-specific mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The mixture did not simply suppress all stress hormones.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jezova2005] Subchronic treatment with amino acid mixture of L-lysine and L-arginine modifies neuroendocrine activation during psychosocial stress in subjects with high trait anxiety (2005). https://pubmed.ncbi.nlm.nih.gov/16117182/ DOI: 10.1080/10284150500162937","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Blood stress hormones and cardiovascular measurements","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"84e9714a-535c-5c98-828b-ecf831ef9824","evidence_kind":"source_excerpt","locator":"Lines 739-747","start_line":739,"end_line":747,"excerpt":"### lysine-arginine-stress-hormones\nIn 29 high-trait-anxiety participants, lysine plus arginine increased stress-evoked ACTH, cortisol, adrenaline, and noradrenaline responses without changing heart-rate or blood-pressure responses.\nPlain language: The mixture did not simply suppress all stress hormones.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Blood stress hormones and cardiovascular measurements\nexperimental_model: Ten-day randomized trial; 3 g/day of each amino acid, followed by public-speaking stress\nlimitations: Small combination trial; authors' proposed normalization is an interpretation, not a proven lysine-specific mechanism.\n[jezova2005] Subchronic treatment with amino acid mixture of L-lysine and L-arginine modifies neuroendocrine activation during psychosocial stress in subjects with high trait anxiety (2005). https://pubmed.ncbi.nlm.nih.gov/16117182/ DOI: 10.1080/10284150500162937","model_system":"Ten-day randomized trial; 3 g/day of each amino acid, followed by public-speaking stress","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [jezova2005] Subchronic treatment with amino acid mixture of L-lysine and L-arginine modifies neuroendocrine activation during psychosocial stress in subjects with high trait anxiety (2005). https://pubmed.ncbi.nlm.nih.gov/16117182/ DOI: 10.1080/10284150500162937","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"460b694b-0401-5c0d-a935-0e3366b6db49","title":"Lysine-related interventions do not uniformly lower stress-hormone responses","kind":"context_difference","status":"open","why":"The trials differ in sex, diet, stressor, baseline anxiety, and arginine cointervention. Increased and decreased evoked hormones are not directly contradictory observations; neither supports a universal cortisol-lowering claim.","resolution":"Unresolved; needs review.","created_at":"2026-09-17 07:46:03","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/460b694b-0401-5c0d-a935-0e3366b6db49","sides":[{"conflict_id":"460b694b-0401-5c0d-a935-0e3366b6db49","ordinal":0,"label":"Lysine fortification reduces anxiety and lessens stress in family members in economically weak communities in Northwest Syria","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":699,"end_line":707,"quote":"### syria-stress-cortisol\nIn the Syrian trial, lysine fortification reduced the cortisol response associated with blood drawing in females, without the same effect in males.\nPlain language: The measured response to a specific stressor differed by sex.\nCondition category: nutrient_deficiency\norganism: Homo sapiens\ntissue_or_cell_type: Blood cortisol under venipuncture stress\nexperimental_model: Randomized household fortification trial\nlimitations: Prestress baseline cortisol was not measured; not evidence of universal cortisol lowering.\n[smriga2004] Lysine fortification reduces anxiety and lessens stress in family members in economically weak communities in Northwest Syria (2004). https://pmc.ncbi.nlm.nih.gov/articles/PMC420386/ DOI: 10.1073/pnas.0402550101","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["79bb93a4-3b08-530b-991b-5972c13a8c8b"]},{"conflict_id":"460b694b-0401-5c0d-a935-0e3366b6db49","ordinal":1,"label":"Subchronic treatment with amino acid mixture of L-lysine and L-arginine modifies neuroendocrine activation during psychosocial stress in subjects with high trait anxiety","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","start_line":739,"end_line":747,"quote":"### lysine-arginine-stress-hormones\nIn 29 high-trait-anxiety participants, lysine plus arginine increased stress-evoked ACTH, cortisol, adrenaline, and noradrenaline responses without changing heart-rate or blood-pressure responses.\nPlain language: The mixture did not simply suppress all stress hormones.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Blood stress hormones and cardiovascular measurements\nexperimental_model: Ten-day randomized trial; 3 g/day of each amino acid, followed by public-speaking stress\nlimitations: Small combination trial; authors' proposed normalization is an interpretation, not a proven lysine-specific mechanism.\n[jezova2005] Subchronic treatment with amino acid mixture of L-lysine and L-arginine modifies neuroendocrine activation during psychosocial stress in subjects with high trait anxiety (2005). https://pubmed.ncbi.nlm.nih.gov/16117182/ DOI: 10.1080/10284150500162937","source_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","source_title":"L-Lysine: mechanism-first literature curation (2026-09-17)","claim_ids":["4611ee3b-522a-5916-9760-f2ca33e3bba9"]}]}],"corrections":[],"research":null}