{"id":"46019e42-3c98-5560-b064-ebc36090a319","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-brain-ascorbate","predicate":"reduces","statement":"High-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8140beb9-6baa-5a1a-8bea-7e574c40b348","mechanism_event_label":"High-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"0a6d34e8-f4a0-5325-bc39-c817173e0ac9","slug":"rat-brain-ascorbic-acid-content","display_name":"Rat brain ascorbic acid content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8140beb9-6baa-5a1a-8bea-7e574c40b348","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-brain-ascorbate-event","event_type":"observed_relationship","label":"High-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.","description":"High-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a6d34e8-f4a0-5325-bc39-c817173e0ac9","slug":"rat-brain-ascorbic-acid-content","display_name":"Rat brain ascorbic acid content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 700 mg/kg body weight for 2 weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"2 treatment weeks; endpoint tissue analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male Wistar rats; six per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Very high animal exposure. Results section reports lower ascorbic acid with no significant GSH or MDA change from tartrazine alone; broader abstract/discussion wording should not replace these results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Male Wistar rats; six per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"High-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"High-Dose Aspirin Reverses Tartrazine-Induced Cell Growth Dysregulation Independent of p53 Signaling and Antioxidant Mechanisms in Rat Brain. (2019). https://pubmed.ncbi.nlm.nih.gov/31032366/ DOI: 10.1155/2019/9096404","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral tartrazine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Brain tissue biochemical and histological endpoints","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c76d2f07-5078-539a-9aac-ff4a1bb97dab","evidence_kind":"source_excerpt","locator":"Lines 358-367","start_line":358,"end_line":367,"excerpt":"## tartrazine-brain-ascorbate\nHigh-dose oral tartrazine lowered measured brain ascorbic acid in the rat experiment.\nModel/species: Male Wistar rats; six per group\nTissue: Brain tissue biochemical and histological endpoints\nExposure: Tartrazine 700 mg/kg body weight for 2 weeks\nRoute: Oral tartrazine\nDuration: 2 treatment weeks; endpoint tissue analysis\nLimits: Very high animal exposure. Results section reports lower ascorbic acid with no significant GSH or MDA change from tartrazine alone; broader abstract/discussion wording should not replace these results.\nPrimary reference: High-Dose Aspirin Reverses Tartrazine-Induced Cell Growth Dysregulation Independent of p53 Signaling and Antioxidant Mechanisms in Rat Brain. (2019). https://pubmed.ncbi.nlm.nih.gov/31032366/ DOI: 10.1155/2019/9096404\nAccess: Primary full-text methods/results and metadata.","model_system":"Male Wistar rats; six per group","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}