{"id":"45f7aef3-5891-56af-bc54-1e4100e61659","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zinc-calcium-signal","predicate":"sustained","statement":"Higher extracellular zinc sustained the cytoplasmic calcium signal after T-cell activation; initial calcium rises were similar, then the signals diverged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3857a520-38e3-51ad-b34e-0ad98c0a20ea","mechanism_event_label":"Zinc availability affected how long the calcium signal lasted.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"80d3e7fb-8400-5cf8-a0d0-ab3fadb20286","slug":"t-cell-calcium-signal","display_name":"T-cell cytoplasmic calcium signal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3857a520-38e3-51ad-b34e-0ad98c0a20ea","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zinc-calcium-signal-event","event_type":"biochemical_relationship","label":"Zinc availability affected how long the calcium signal lasted.","description":"Higher extracellular zinc sustained the cytoplasmic calcium signal after T-cell activation; initial calcium rises were similar, then the signals diverged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"signaling_ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"517e6e15-f4f2-579f-b4e8-b5a5a40345f1","slug":"slc39a6","display_name":"Human zinc transporter ZIP6 / SLC39A6","entity_type_key":"protein"},"role":"upstream_transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"80d3e7fb-8400-5cf8-a0d0-ab3fadb20286","slug":"t-cell-calcium-signal","display_name":"T-cell cytoplasmic calcium signal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"calcium ion (signaling_ion); Human zinc transporter ZIP6 / SLC39A6 (upstream_transporter)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/21422171.txt\", \"locator\": \"Results: zinc availability and TCR signaling; Figure 4e calcium time course\", \"file_sha256\": \"4d36b4fdeeff83b602b0532796814981ee45409241fe40fcd8f7bc1da8beff19\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human T-cell receptor signaling study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Receptor activation, extracellular zinc availability and ZIP6 suppression comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture zinc availability and local signals cannot be mapped directly to oral doses or plasma thresholds. Calcium fluorescence tracks the signal rather than proving one specific calcium channel mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Zinc availability affected how long the calcium signal lasted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. (2011). https://pubmed.ncbi.nlm.nih.gov/21422171/ DOI: 10.1084/jem.20100031","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CD4 T cells at the receptor-contact region","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9a383ea9-0209-5bce-b09b-2b674b8b5efb","evidence_kind":"source_excerpt","locator":"Lines 1055-1067","start_line":1055,"end_line":1067,"excerpt":"### zn-sig-zinc-calcium-signal\nHigher extracellular zinc sustained the cytoplasmic calcium signal after T-cell activation; initial calcium rises were similar, then the signals diverged.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Zinc availability affected how long the calcium signal lasted.\norganism: Homo sapiens\ntissue_or_cell_type: CD4 T cells at the receptor-contact region\nexperimental_model: Primary human T-cell receptor signaling study\nlimitations: Culture zinc availability and local signals cannot be mapped directly to oral doses or plasma thresholds. Calcium fluorescence tracks the signal rather than proving one specific calcium channel mechanism.\nexposure: Receptor activation, extracellular zinc availability and ZIP6 suppression comparisons.\ncross_nutrient: calcium ion (signaling_ion); Human zinc transporter ZIP6 / SLC39A6 (upstream_transporter)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/21422171.txt\", \"locator\": \"Results: zinc availability and TCR signaling; Figure 4e calcium time course\", \"file_sha256\": \"4d36b4fdeeff83b602b0532796814981ee45409241fe40fcd8f7bc1da8beff19\"}\n[zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. (2011). https://pubmed.ncbi.nlm.nih.gov/21422171/ DOI: 10.1084/jem.20100031","model_system":"Primary human T-cell receptor signaling study","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. (2011). https://pubmed.ncbi.nlm.nih.gov/21422171/ DOI: 10.1084/jem.20100031","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}