{"id":"44e93d46-a20d-5c9d-a660-241b5f4aee73","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-camkii-oxidation","predicate":"undergoes_regulatory_oxidation_in","statement":"Oxidation of paired CaMKII regulatory methionines sustained kinase activity after calcium/calmodulin was removed.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"95809b96-5174-5004-85f6-9765e0e619a5","mechanism_event_label":"Oxidation can leave a signaling protein switched on.","subject":{"id":"c5448053-5edf-5d0f-8027-a69cc580e030","slug":"protein-methionine","display_name":"Protein-bound methionine","entity_type_key":"chemical_species"},"object":{"id":"32b99bd0-bfb8-56dc-9f96-4df641c65a24","slug":"oxidized-camkii-regulatory-methionines","display_name":"Oxidized regulatory methionines in the CaMKII study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"95809b96-5174-5004-85f6-9765e0e619a5","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-camkii-oxidation-event","event_type":"observed_relationship","label":"Oxidation can leave a signaling protein switched on.","description":"Oxidation of paired CaMKII regulatory methionines sustained kinase activity after calcium/calmodulin was removed.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c5448053-5edf-5d0f-8027-a69cc580e030","slug":"protein-methionine","display_name":"Protein-bound methionine","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32b99bd0-bfb8-56dc-9f96-4df641c65a24","slug":"oxidized-camkii-regulatory-methionines","display_name":"Oxidized regulatory methionines in the CaMKII study","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c91a8ec8-1efa-5253-bb4c-435b8575f1cd","slug":"camkii","display_name":"Ca2+/calmodulin-dependent protein kinase II family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical kinase assays with cardiomyocyte and mouse experiments; exact purified construct species not resolved here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Initial calcium/calmodulin exposure and sustained autonomous activity are distinct; not a dietary methionine effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Oxidation can leave a signaling protein switched on.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18455987/ · DOI 10.1016/j.cell.2008.02.048","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6147bb2b-1d59-5c10-bf54-143af281e651","evidence_kind":"source_excerpt","locator":"Lines 332-338","start_line":332,"end_line":338,"excerpt":"## methionine-camkii-oxidation\nOxidation can leave a signaling protein switched on.\nOxidation of paired CaMKII regulatory methionines sustained kinase activity after calcium/calmodulin was removed.\nModel: Biochemical kinase assays with cardiomyocyte and mouse experiments; exact purified construct species not resolved here.\nLimitations: Initial calcium/calmodulin exposure and sustained autonomous activity are distinct; not a dietary methionine effect.\nEvidence access: Primary abstract\nA dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18455987/ · DOI 10.1016/j.cell.2008.02.048","model_system":"Biochemical kinase assays with cardiomyocyte and mouse experiments; exact purified construct species not resolved here.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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