{"id":"44da6a30-0e80-5475-9630-5c2a03d3a639","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-vrk1-binding","predicate":"binds","statement":"Pull-down, SPR and NMR experiments supported direct luteolin binding near the human VRK1 catalytic domain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c066893b-28c2-5ea1-9c77-d4f543ac8c77","mechanism_event_label":"Physical binding evidence accompanies the kinase result.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"2c01de74-8b26-5489-824c-6b08d9958cbf","slug":"vrk1","display_name":"Human vaccinia-related kinase 1 / VRK1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c066893b-28c2-5ea1-9c77-d4f543ac8c77","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-vrk1-binding-event","event_type":"observed_relationship","label":"Physical binding evidence accompanies the kinase result.","description":"Pull-down, SPR and NMR experiments supported direct luteolin binding near the human VRK1 catalytic domain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2c01de74-8b26-5489-824c-6b08d9958cbf","slug":"vrk1","display_name":"Human vaccinia-related kinase 1 / VRK1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant protein and human-cell lysates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No crystal-defined pose; published SPR table constants are not used here as a validated affinity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Physical binding evidence accompanies the kinase result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin suppresses cancer cell proliferation by targeting vaccinia-related kinase 1. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25310002/ · DOI 10.1371/journal.pone.0109655","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eff8a524-dd77-5c5f-bd73-dbcbfde31185","evidence_kind":"source_excerpt","locator":"Lines 348-354","start_line":348,"end_line":354,"excerpt":"## luteolin-vrk1-binding\nPhysical binding evidence accompanies the kinase result.\nPull-down, SPR and NMR experiments supported direct luteolin binding near the human VRK1 catalytic domain.\nModel: Recombinant protein and human-cell lysates.\nLimitations: No crystal-defined pose; published SPR table constants are not used here as a validated affinity.\nEvidence access: Primary full text\nLuteolin suppresses cancer cell proliferation by targeting vaccinia-related kinase 1. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25310002/ · DOI 10.1371/journal.pone.0109655","model_system":"Recombinant protein and human-cell lysates.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}