{"id":"44c03b4a-da35-5268-9abd-d89bd7b7af56","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-smit1-pi-preserved","predicate":"loss_did_not_decrease","statement":"Despite severely reduced brain myo-inositol, measured brain phosphatidylinositol was not decreased in SMIT1-null mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cafb1c73-0077-5625-802f-70f6dc627c2c","mechanism_event_label":"The free pool and the total membrane-lipid pool did not fall together.","subject":{"id":"7ee41a04-bc5c-53e9-a798-16c5214d8854","slug":"mouse-slc5a3","display_name":"Mouse Slc5a3 (SMIT1)","entity_type_key":"protein"},"object":{"id":"ae758982-4634-5f3a-b9c7-dbc58b744484","slug":"phosphatidylinositol","display_name":"Phosphatidylinositol (PI)","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"cafb1c73-0077-5625-802f-70f6dc627c2c","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-smit1-pi-preserved-event","event_type":"biochemical_relationship","label":"The free pool and the total membrane-lipid pool did not fall together.","description":"Despite severely reduced brain myo-inositol, measured brain phosphatidylinositol was not decreased in SMIT1-null mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7ee41a04-bc5c-53e9-a798-16c5214d8854","slug":"mouse-slc5a3","display_name":"Mouse Slc5a3 (SMIT1)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae758982-4634-5f3a-b9c7-dbc58b744484","slug":"phosphatidylinositol","display_name":"Phosphatidylinositol (PI)","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/15110328.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"efa2c78237254c73512bf946f2600f4ead3f1e510cda84a06327906d4663d3ee\", \"start_char\": 0, \"end_char\": 1202, \"text_sha256\": \"efa2c78237254c73512bf946f2600f4ead3f1e510cda84a06327906d4663d3ee\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transporter knockout and brain chemical analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SMIT1-null mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Severe genetic transport failure is not dietary deficiency. Unchanged total PI does not rule out local phosphoinositide abnormalities. An indexed erratum exists (10.1016/j.ymgme.2004.06.012); its content was not retrievable during this curation, so these findings remain provisional reports from the indexed original abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The free pool and the total membrane-lipid pool did not fall together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p15110328] Phosphoinositide deficiency due to inositol depletion is not a mechanism of lithium action in brain. (2004). https://pubmed.ncbi.nlm.nih.gov/15110328/ DOI: 10.1016/j.ymgme.2004.02.002","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"91ee211f-a6fe-53c9-8137-982b4edfbec3","evidence_kind":"source_excerpt","locator":"Lines 444-455","start_line":444,"end_line":455,"excerpt":"### ino-smit1-pi-preserved\nDespite severely reduced brain myo-inositol, measured brain phosphatidylinositol was not decreased in SMIT1-null mice.\nCondition category: machinery_impairment\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The free pool and the total membrane-lipid pool did not fall together.\norganism: Mus musculus\ntissue_or_cell_type: Brain\nexperimental_model: Transporter knockout and brain chemical analysis\nlimitations: Severe genetic transport failure is not dietary deficiency. Unchanged total PI does not rule out local phosphoinositide abnormalities. An indexed erratum exists (10.1016/j.ymgme.2004.06.012); its content was not retrievable during this curation, so these findings remain provisional reports from the indexed original abstract.\nexposure: SMIT1-null mice\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/15110328.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"efa2c78237254c73512bf946f2600f4ead3f1e510cda84a06327906d4663d3ee\", \"start_char\": 0, \"end_char\": 1202, \"text_sha256\": \"efa2c78237254c73512bf946f2600f4ead3f1e510cda84a06327906d4663d3ee\"}\n[ino-p15110328] Phosphoinositide deficiency due to inositol depletion is not a mechanism of lithium action in brain. (2004). https://pubmed.ncbi.nlm.nih.gov/15110328/ DOI: 10.1016/j.ymgme.2004.02.002","model_system":"Transporter knockout and brain chemical analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p15110328] Phosphoinositide deficiency due to inositol depletion is not a mechanism of lithium action in brain. (2004). https://pubmed.ncbi.nlm.nih.gov/15110328/ DOI: 10.1016/j.ymgme.2004.02.002","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}