{"id":"44be8820-36d1-5947-8aff-ee1d9b4ddd29","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-sit1-uptake","predicate":"imports","statement":"Human SIT1/SLC6A20 functioned as a sodium-dependent proline transporter when expressed for functional testing.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f2594c87-a9d5-5190-916e-90a24ce8c7ba","mechanism_event_label":"Cell entry depends on transport machinery and ion gradients.","subject":{"id":"01b4d4c1-f1a8-59e3-84e3-fb851d5ddf46","slug":"slc6a20","display_name":"Human imino-acid transporter / SLC6A20","entity_type_key":"protein"},"object":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f2594c87-a9d5-5190-916e-90a24ce8c7ba","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-sit1-uptake-event","event_type":"observed_relationship","label":"Cell entry depends on transport machinery and ion gradients.","description":"Human SIT1/SLC6A20 functioned as a sodium-dependent proline transporter when expressed for functional testing.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"01b4d4c1-f1a8-59e3-84e3-fb851d5ddf46","slug":"slc6a20","display_name":"Human imino-acid transporter / SLC6A20","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SIT1 heterologous expression in the mammalian transporter identification study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat concentration-response and ion-substitution details are recorded separately rather than assumed identical in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Cell entry depends on transport machinery and ion gradients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15632147/ · DOI 10.1074/jbc.M413027200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Functioned as a sodium-dependent proline transporter, which is inward.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Functioned as a sodium-dependent proline transporter, which is inward.","entity":null}],"evidence":[{"id":"a2bcccc6-9047-5f6f-8c63-587b159de7c3","evidence_kind":"source_excerpt","locator":"Lines 166-172","start_line":166,"end_line":172,"excerpt":"## l-proline-sit1-uptake\nCell entry depends on transport machinery and ion gradients.\nHuman SIT1/SLC6A20 functioned as a sodium-dependent proline transporter when expressed for functional testing.\nModel: Human SIT1 heterologous expression in the mammalian transporter identification study.\nLimitations: Rat concentration-response and ion-substitution details are recorded separately rather than assumed identical in humans.\nEvidence access: Primary abstract\nIdentification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15632147/ · DOI 10.1074/jbc.M413027200","model_system":"Human SIT1 heterologous expression in the mammalian transporter identification study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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