{"id":"44b396d8-0570-560e-b6c7-03cfb8f1d01a","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-synthesis","predicate":"synthesizes","statement":"Purified recombinant human CARNS1 joined beta-alanine and L-histidine to form carnosine in an ATP-containing assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fdebb65f-cc4f-5ead-879c-161b09bf8b61","mechanism_event_label":"Two building blocks are assembled by a specific enzyme.","subject":{"id":"dda068ca-005f-50f4-9001-42fe79759324","slug":"human-carns1","display_name":"Human carnosine synthase / CARNS1 (ATPGD1)","entity_type_key":"protein"},"object":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fdebb65f-cc4f-5ead-879c-161b09bf8b61","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-synthesis-event","event_type":"observed_relationship","label":"Two building blocks are assembled by a specific enzyme.","description":"Purified recombinant human CARNS1 joined beta-alanine and L-histidine to form carnosine in an ATP-containing assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dda068ca-005f-50f4-9001-42fe79759324","slug":"human-carns1","display_name":"Human carnosine synthase / CARNS1 (ATPGD1)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"288f19a6-62f0-51fe-8e6d-366388b6b16d","slug":"beta-alanine","display_name":"Beta-alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"24904faf-f4b4-5e5a-a2e6-3549fb7a2a2e","slug":"mg-atp","display_name":"Magnesium-ATP complex","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and full-text enzyme methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enzyme expressed in HEK293T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An enzyme assay does not define dietary requirements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; 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