{"id":"44a4109d-6845-57d4-af96-d44b8c4c4ced","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-redox-boundary","predicate":"deletion_did_not_increase","statement":"Under the tested culture conditions, NADK2 deletion did not increase measured mitochondrial oxidative stress or disrupt mitochondrial folate pathway activity despite reduced proline synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9c62b740-268c-57d9-aa46-25e64c0cd85c","mechanism_event_label":"Losing this cofactor pool did not shut down every pathway that might be connected to it.","subject":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"object":{"id":"88e29f1a-d313-54ae-879c-d5e2ee266981","slug":"human-nadk2-null-oxidative-stress","display_name":"Oxidative-stress readouts in NADK2-deleted human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9c62b740-268c-57d9-aa46-25e64c0cd85c","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-redox-boundary-event","event_type":"observed_relationship","label":"Losing this cofactor pool did not shut down every pathway that might be connected to it.","description":"Under the tested culture conditions, NADK2 deletion did not increase measured mitochondrial oxidative stress or disrupt mitochondrial folate pathway activity despite reduced proline synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"88e29f1a-d313-54ae-879c-d5e2ee266981","slug":"human-nadk2-null-oxidative-stress","display_name":"Oxidative-stress readouts in NADK2-deleted human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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