{"id":"44498903-8bfb-5a56-9889-de3e8b435bda","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-breast-mpst","predicate":"increases_tested_expression_of","statement":"At 4.50 mM for 24 hours, SAC increased MPST gene expression in both MCF-7 and MDA-MB-231 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ba21a235-257f-5aa8-a3db-85f640397380","mechanism_event_label":"An enzyme involved in sulfur metabolism changed expression.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"bd69cbdf-a220-5981-b89f-c31b53f25f40","slug":"mpst","display_name":"Human mercaptopyruvate sulfurtransferase / MPST","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ba21a235-257f-5aa8-a3db-85f640397380","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-breast-mpst-event","event_type":"observed_relationship","label":"An enzyme involved in sulfur metabolism changed expression.","description":"At 4.50 mM for 24 hours, SAC increased MPST gene expression in both MCF-7 and MDA-MB-231 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bd69cbdf-a220-5981-b89f-c31b53f25f40","slug":"mpst","display_name":"Human mercaptopyruvate sulfurtransferase / MPST","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"2.24, 3.37 or 4.50 mM SAC","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"2, 4, 6, 8 or 24 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC versus untreated cell controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MCF-7 and MDA-MB-231 breast adenocarcinoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; 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mRNA does not establish enzyme flux or net H2S production.\nEvidence access: Primary abstract\n[38397425] S-Allyl-L-Cysteine Affects Cell Proliferation and Expression of H2S-Synthetizing Enzymes in MCF-7 and MDA-MB-231 Adenocarcinoma Cell Lines. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38397425/ · DOI 10.3390/biom14020188\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"MCF-7 and MDA-MB-231 breast adenocarcinoma cells\", \"dose\": \"2.24, 3.37 or 4.50 mM SAC\", \"duration\": \"2, 4, 6, 8 or 24 hours\", \"route\": \"Cell culture\", \"experimental_comparison\": \"SAC versus untreated cell controls\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"MCF-7 and MDA-MB-231 breast adenocarcinoma cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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