{"id":"4435a88f-b7e7-5a7d-a323-631c491f21c7","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-bco2-trans-null","predicate":"did_not_detectably_cleave","statement":"All-trans lycopene was not cleaved by ferret BCO2 under the reported conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"92a1b51e-48e8-5a70-9d25-0e3379a7c8c4","mechanism_event_label":"The straight form did not behave like the cis forms in this enzyme assay.","subject":{"id":"cf0a614d-af35-5332-880a-f92c9466a294","slug":"ferret-bco2","display_name":"Ferret beta-carotene oxygenase 2 / BCO2","entity_type_key":"protein"},"object":{"id":"4a88840d-be05-5442-9938-a1da9df4d385","slug":"all-trans-lycopene","display_name":"All-trans-lycopene / all-E-lycopene","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"92a1b51e-48e8-5a70-9d25-0e3379a7c8c4","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-bco2-trans-null-event","event_type":"biochemical_relationship","label":"The straight form did not behave like the cis forms in this enzyme assay.","description":"All-trans lycopene was not cleaved by ferret BCO2 under the reported conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cf0a614d-af35-5332-880a-f92c9466a294","slug":"ferret-bco2","display_name":"Ferret beta-carotene oxygenase 2 / BCO2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4a88840d-be05-5442-9938-a1da9df4d385","slug":"all-trans-lycopene","display_name":"All-trans-lycopene / all-E-lycopene","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/16672231.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\", \"start_char\": 0, \"end_char\": 1967, \"text_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant ferret enzyme and tissue-fraction metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5-cis, 13-cis and all-trans lycopene; iron; NAD+ or NADH","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ferret experiments do not quantify human cleavage flux or predict supplement response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Ferret","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The straight form did not behave like the cis forms in this enzyme assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p16672231] The biochemical characterization of ferret carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in vitro and in vivo. (2006). https://pubmed.ncbi.nlm.nih.gov/16672231/ DOI: 10.1074/jbc.m512095200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"BCO2 enzyme; hepatic fractions and lung","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e8ada239-fd55-5c31-b5a1-73524169f4f4","evidence_kind":"source_excerpt","locator":"Lines 312-323","start_line":312,"end_line":323,"excerpt":"### lycopene-bco2-trans-null\nAll-trans lycopene was not cleaved by ferret BCO2 under the reported conditions.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The straight form did not behave like the cis forms in this enzyme assay.\norganism: Ferret\ntissue_or_cell_type: BCO2 enzyme; hepatic fractions and lung\nexperimental_model: Recombinant ferret enzyme and tissue-fraction metabolism\nlimitations: Ferret experiments do not quantify human cleavage flux or predict supplement response.\nexposure: 5-cis, 13-cis and all-trans lycopene; iron; NAD+ or NADH\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/16672231.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\", \"start_char\": 0, \"end_char\": 1967, \"text_sha256\": \"135695c9d3106469d8d64ec0806ba5bf048f0f1df6f3e3cd53e838e50b960445\"}\n[lycopene-p16672231] The biochemical characterization of ferret carotene-9',10'-monooxygenase catalyzing cleavage of carotenoids in vitro and in vivo. 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