{"id":"441134ff-2370-5764-9799-318cb2370677","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-tcell-pp2a-suppression","predicate":"inhibits","statement":"Elevated intracellular K suppressed TCR-driven AKT-mTOR signaling through a PP2A-dependent process.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a3d1f1c2-10df-55de-a9ac-9a4508e79e25","mechanism_event_label":"Local potassium accumulation dampened T-cell activation signals.","subject":{"id":"8c7b3fef-22fb-5b65-9b51-59f776234bc3","slug":"intracellular-potassium-concentration","display_name":"Intracellular potassium concentration","entity_type_key":"cellular_process"},"object":{"id":"a32e7013-dfef-562f-877b-25d6e5fd4837","slug":"t-cell-akt-mtor-signaling","display_name":"T-cell AKT-mTOR signaling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a3d1f1c2-10df-55de-a9ac-9a4508e79e25","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-tcell-pp2a-suppression-event","event_type":"biochemical_relationship","label":"Local potassium accumulation dampened T-cell activation signals.","description":"Elevated intracellular K suppressed TCR-driven AKT-mTOR signaling through a PP2A-dependent process.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a1f0e3dd-7853-5c6e-80dc-662c1a2527b0","slug":"pp2a-complexes","display_name":"Protein phosphatase 2A complexes","entity_type_key":"protein_family"},"role":"required phosphatase activity","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a13cb592-c939-5f92-9206-d774248802fc","slug":"akt-proteins","display_name":"AKT serine/threonine kinase family","entity_type_key":"protein_family"},"role":"signaling kinase","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"role":"downstream complex","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8c7b3fef-22fb-5b65-9b51-59f776234bc3","slug":"intracellular-potassium-concentration","display_name":"Intracellular potassium concentration","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a32e7013-dfef-562f-877b-25d6e5fd4837","slug":"t-cell-akt-mtor-signaling","display_name":"T-cell AKT-mTOR signaling","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"High-K mouse/human T-cell cultures; PP2A perturbations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"PP2A dependence does not demonstrate direct K binding; no diet manipulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse and human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Local potassium accumulation dampened T-cell activation signals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[eil-2016-tcells] Ionic immune suppression within the tumour microenvironment limits T cell effector function (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC5204372/ DOI: 10.1038/nature19364","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"669ba4ff-5d84-55eb-83ef-7cd09955be0e","evidence_kind":"source_excerpt","locator":"Lines 902-911","start_line":902,"end_line":911,"excerpt":"### k-tcell-pp2a-suppression\nElevated intracellular K suppressed TCR-driven AKT-mTOR signaling through a PP2A-dependent process.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Local potassium accumulation dampened T-cell activation signals.\norganism: Mouse and human\ntissue_or_cell_type: T cells\nexperimental_model: High-K mouse/human T-cell cultures; PP2A perturbations.\nlimitations: PP2A dependence does not demonstrate direct K binding; no diet manipulation.\n[eil-2016-tcells] Ionic immune suppression within the tumour microenvironment limits T cell effector function (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC5204372/ DOI: 10.1038/nature19364","model_system":"High-K mouse/human T-cell cultures; PP2A perturbations.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [eil-2016-tcells] Ionic immune suppression within the tumour microenvironment limits T cell effector function (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC5204372/ DOI: 10.1038/nature19364","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}