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purified/reconstituted metabolism and tissue assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Incubation with 4-hydroxy-all-trans-retinoic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This assay does not assign every downstream metabolite a structure or biological inactivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens recombinant protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"outcome","value_text":"CYP26A1 further metabolized 4-hydroxy-all-trans-retinoic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Oxidation continues beyond the first hydroxylated product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-topletz-2012] Comparison of the function and expression of CYP26A1 and CYP26B1, the two retinoic acid hydroxylases (2012). https://pubmed.ncbi.nlm.nih.gov/22020119/ DOI: 10.1016/j.bcp.2011.10.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Reconstituted enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e0a7a0c1-a6ff-5916-97c3-5b84990bed91","evidence_kind":"source_excerpt","locator":"Lines 630-642","start_line":630,"end_line":642,"excerpt":"### va-cyp26a1-secondary-metabolism\nCYP26A1 further metabolized 4-hydroxy-all-trans-retinoic acid.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Oxidation continues beyond the first hydroxylated product.\norganism: Homo sapiens recombinant protein\ntissue_or_cell_type: Reconstituted enzyme\nexperimental_model: Human CYP26A1/CYP26B1 expressed in insect cells; purified/reconstituted metabolism and tissue assays.\nlimitations: This assay does not assign every downstream metabolite a structure or biological inactivity.\nexposure: Incubation with 4-hydroxy-all-trans-retinoic acid.\noutcome: CYP26A1 further metabolized 4-hydroxy-all-trans-retinoic acid.\nevidence_location: Abstract\n[va-topletz-2012] Comparison of the function and expression of CYP26A1 and CYP26B1, the two retinoic acid hydroxylases (2012). https://pubmed.ncbi.nlm.nih.gov/22020119/ DOI: 10.1016/j.bcp.2011.10.007","model_system":"Human CYP26A1/CYP26B1 expressed in insect cells; purified/reconstituted metabolism and tissue assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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