{"id":"43503776-1498-5c75-8dc3-6950fd0ffe77","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-trna-intermediate","predicate":"produces","statement":"The t6A pathway first forms threonylcarbamoyladenylate from threonine, bicarbonate and ATP; OSGEPL1 then transfers the threonylcarbamoyl group to mitochondrial tRNA A37.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"68f3dc11-2a1c-58d7-afff-bb687c7af0e0","mechanism_event_label":"An activated intermediate links nutrient supply to an RNA modification.","subject":{"id":"3114b5d2-04d2-59d6-88c3-c04eb28aeeff","slug":"yrdc","display_name":"Human threonylcarbamoyladenylate synthase / YRDC","entity_type_key":"protein"},"object":{"id":"cf664fc7-c0f3-589c-a191-27e1c2276175","slug":"threonylcarbamoyl-amp","display_name":"L-Threonylcarbamoyladenylate / TC-AMP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"68f3dc11-2a1c-58d7-afff-bb687c7af0e0","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-trna-intermediate-event","event_type":"observed_relationship","label":"An activated intermediate links nutrient supply to an RNA modification.","description":"The t6A pathway first forms threonylcarbamoyladenylate from threonine, bicarbonate and ATP; 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the 2024 study primarily tests loss of the downstream enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An activated intermediate links nutrient supply to an RNA modification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Multifaceted roles of t6A biogenesis in efficiency and fidelity of mitochondrial gene expression. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38227555/ · DOI 10.1093/nar/gkae013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b001664a-10eb-56dd-bf75-7d2ef424d0eb","evidence_kind":"source_excerpt","locator":"Lines 130-136","start_line":130,"end_line":136,"excerpt":"## l-threonine-trna-intermediate\nAn activated intermediate links nutrient supply to an RNA modification.\nThe t6A pathway first forms threonylcarbamoyladenylate from threonine, bicarbonate and ATP; OSGEPL1 then transfers the threonylcarbamoyl group to mitochondrial tRNA A37.\nModel: Primary paper pathway description supporting human mitochondrial experiments.\nLimitations: The reaction sequence is described in the primary paper introduction; the 2024 study primarily tests loss of the downstream enzyme.\nEvidence access: Primary abstract and accessible primary-paper pathway description\nMultifaceted roles of t6A biogenesis in efficiency and fidelity of mitochondrial gene expression. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38227555/ · DOI 10.1093/nar/gkae013","model_system":"Primary paper pathway description supporting human mitochondrial experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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