{"id":"43371c34-2bd7-5c43-94fe-4db3e9bf80ac","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-sars-cov-2-cell-culture","predicate":"reduces_in_culture","statement":"A single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f23daf14-19d6-55a0-a067-da88acb176f1","mechanism_event_label":"A single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"75b52944-6cca-5633-a374-453ce25b2032","slug":"sars-cov-2-viral-rna","display_name":"SARS-CoV-2 viral RNA in Vero-hSLAM culture","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f23daf14-19d6-55a0-a067-da88acb176f1","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-sars-cov-2-cell-culture-event","event_type":"observed_relationship","label":"A single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.","description":"A single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"75b52944-6cca-5633-a374-453ce25b2032","slug":"sars-cov-2-viral-rna","display_name":"SARS-CoV-2 viral RNA in Vero-hSLAM culture","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"48 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Vero-hSLAM cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Single ivermectin addition two hours post infection","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a cell-culture result in a monkey kidney line, and the authors state only that it warrants further investigation for possible benefits in humans. The concentration relationship to human plasma exposure is not addressed in the report and remains unresolved here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Vero-hSLAM cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The FDA-approved drug ivermectin inhibits the replication of SARS-CoV-2 in vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32251768/ DOI: 10.1016/j.antiviral.2020.104787","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Viral RNA","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"21b4d793-3715-57ad-aa72-4e2433864a44","evidence_kind":"source_excerpt","locator":"Lines 167-176","start_line":167,"end_line":176,"excerpt":"## ivermectin-sars-cov-2-cell-culture\nA single addition of ivermectin to SARS-CoV-2-infected Vero-hSLAM cells two hours after infection produced approximately a five thousand fold reduction in viral RNA at forty-eight hours.\nModel/species: Vero-hSLAM cell culture\nTissue/system: Viral RNA\nExposure: Single ivermectin addition two hours post infection\nRoute: In vitro\nDuration: 48 hours\nLimits: This is a cell-culture result in a monkey kidney line, and the authors state only that it warrants further investigation for possible benefits in humans. The concentration relationship to human plasma exposure is not addressed in the report and remains unresolved here.\nPrimary reference: The FDA-approved drug ivermectin inhibits the replication of SARS-CoV-2 in vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32251768/ DOI: 10.1016/j.antiviral.2020.104787\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"c7e1e0201cbd222f0c52b7981f078936ed39b617d747821ed6daa584c2b964a7","revision_id":"56746c3e-cdec-5b8f-a191-425de1a0a7ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}