{"id":"430a244f-16fa-5920-86e1-a5f6b73fd8f6","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-vitamin-a-withdrawal","predicate":"dietary_depletion_blocks","statement":"Dietary vitamin A depletion caused undifferentiated spermatogonia to accumulate in prepubertal Lrat-null testes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"138ee377-e784-5e15-9e62-baa152b4f867","mechanism_event_label":"Vitamin A shortage can stop early sperm-cell differentiation.","subject":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"object":{"id":"789430ea-0338-54de-b8bf-c6270022971e","slug":"mouse-spermatogonial-differentiation","display_name":"Mouse spermatogonial differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"138ee377-e784-5e15-9e62-baa152b4f867","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-vitamin-a-withdrawal-event","event_type":"biochemical_relationship","label":"Vitamin A shortage can stop early sperm-cell differentiation.","description":"Dietary vitamin A depletion caused undifferentiated spermatogonia to accumulate in prepubertal Lrat-null testes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"role":"sensitizing_storage_defect","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"789430ea-0338-54de-b8bf-c6270022971e","slug":"mouse-spermatogonial-differentiation","display_name":"Mouse spermatogonial differentiation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genotype-by-diet mouse comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Maternal vitamin A-free diet from E0.5 through lactation; neonatal endpoints through P18.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Storage-defective background accelerated depletion; this is not a knockout-only effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin A shortage can stop early sperm-cell differentiation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[li2011] Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. (2011). https://pubmed.ncbi.nlm.nih.gov/20881313/ DOI: 10.1095/biolreprod.110.086157","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"prepubertal testis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c11dc3ab-5345-55a9-85c1-3926000d2648","evidence_kind":"source_excerpt","locator":"Lines 265-276","start_line":265,"end_line":276,"excerpt":"### va-repro-vitamin-a-withdrawal\nDietary vitamin A depletion caused undifferentiated spermatogonia to accumulate in prepubertal Lrat-null testes.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin A shortage can stop early sperm-cell differentiation.\norganism: Mus musculus\ntissue_or_cell_type: prepubertal testis\nexperimental_model: Genotype-by-diet mouse comparison\nlimitations: Storage-defective background accelerated depletion; this is not a knockout-only effect.\nexposure: Maternal vitamin A-free diet from E0.5 through lactation; neonatal endpoints through P18.\ncross_nutrient: false\n[li2011] Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis. 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