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(2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"68294784-85ae-5c28-9df8-2cae9829f784","evidence_kind":"source_excerpt","locator":"Lines 710-721","start_line":710,"end_line":721,"excerpt":"### coq10-coq5-sam\nReconstructed COQ5 catalyzed C-methylation with SAM.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second methylating enzyme links CoQ assembly to methyl-donor availability.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 23488, \"end_char\": 27391, \"text_sha256\": \"7147c9d517477cd7bbec4a155e5f13bc733d294380975977eba11b64517047f3\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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