{"id":"427c6909-4642-5f61-b720-7851444ab5b6","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-berberine-endothelium","predicate":"attenuates","statement":"In HUVEC experiments, berberine reduced vanadyl-acetylacetonate-associated apoptosis, junction/cytoskeleton damage and permeability changes, with lower excessive ERK/Akt phosphorylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"81c0b537-6079-5190-a6ca-0f74087055c1","mechanism_event_label":"Protection from one compound’s cellular toxicity can accompany lower signaling activity.","subject":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"object":{"id":"1892568f-60dd-5f79-ae76-73e3239ffd73","slug":"human-huvec-vanadyl-acac-injury","display_name":"Vanadyl-acetylacetonate injury in human HUVEC cultures","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"81c0b537-6079-5190-a6ca-0f74087055c1","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-berberine-endothelium-event","event_type":"observed_relationship","label":"Protection from one compound’s cellular toxicity can accompany lower signaling activity.","description":"In HUVEC experiments, berberine reduced vanadyl-acetylacetonate-associated apoptosis, junction/cytoskeleton damage and permeability changes, with lower excessive ERK/Akt phosphorylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1892568f-60dd-5f79-ae76-73e3239ffd73","slug":"human-huvec-vanadyl-acac-injury","display_name":"Vanadyl-acetylacetonate injury in human HUVEC cultures","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"664d6382-87fc-577a-9a28-ae713035d2b1","slug":"vanadyl-acetylacetonate","display_name":"Bis(acetylacetonato)oxovanadium(IV)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae89cec3-34fe-5d92-bdcf-b3c745f0f405","slug":"mapk1","display_name":"Human ERK2 / MAPK1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"1d07742f-1a59-5c14-b26b-3f1847be6999","slug":"mapk3","display_name":"Human ERK1 / MAPK3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"a13cb592-c939-5f92-9206-d774248802fc","slug":"akt-proteins","display_name":"AKT serine/threonine kinase family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human endothelial-cell experiments accompanying diabetic-rat study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pathway association does not establish direct inhibition of ERK or Akt by berberine at human exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Protection from one compound’s cellular toxicity can accompany lower signaling activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadyl Acetylacetonate and Berberine Synergistically Ameliorate Diabetes-induced Vascular Dysfunction and Reduce Endothelial Toxicity through ERK and Akt Regulation. · 2026 · https://pubmed.ncbi.nlm.nih.gov/40775562/ · DOI 10.1007/s12011-025-04774-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7771a0db-2593-5572-b523-c1152220b5e5","evidence_kind":"source_excerpt","locator":"Lines 350-356","start_line":350,"end_line":356,"excerpt":"## vanadium-berberine-endothelium\nProtection from one compound’s cellular toxicity can accompany lower signaling activity.\nIn HUVEC experiments, berberine reduced vanadyl-acetylacetonate-associated apoptosis, junction/cytoskeleton damage and permeability changes, with lower excessive ERK/Akt phosphorylation.\nModel: Human endothelial-cell experiments accompanying diabetic-rat study.\nLimitations: Pathway association does not establish direct inhibition of ERK or Akt by berberine at human exposure.\nEvidence access: Primary abstract\nVanadyl Acetylacetonate and Berberine Synergistically Ameliorate Diabetes-induced Vascular Dysfunction and Reduce Endothelial Toxicity through ERK and Akt Regulation. · 2026 · https://pubmed.ncbi.nlm.nih.gov/40775562/ · DOI 10.1007/s12011-025-04774-z","model_system":"Human endothelial-cell experiments accompanying diabetic-rat study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}