{"id":"4245448a-729d-598b-8e71-8c8bf63a0ba4","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-mitoferrin-loss","predicate":"loss_impaired","statement":"Mfrn-null mouse embryonic-stem-cell-derived erythroblasts arrested maturation and had severely impaired incorporation of iron-55 into heme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5f125396-044c-5fbb-b938-e400b713e324","mechanism_event_label":"Plenty of iron elsewhere would not replace this missing mitochondrial transport step.","subject":{"id":"dccf941b-8faf-5cca-b531-b3f08f30804a","slug":"mouse-slc25a37","display_name":"Mouse mitoferrin-1 / Slc25a37","entity_type_key":"protein"},"object":{"id":"1007c432-b01d-5381-8756-60d6c68e072d","slug":"erythroid-heme-synthesis","display_name":"Erythroid heme synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5f125396-044c-5fbb-b938-e400b713e324","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-mitoferrin-loss-event","event_type":"biochemical_relationship","label":"Plenty of iron elsewhere would not replace this missing mitochondrial transport step.","description":"Mfrn-null mouse embryonic-stem-cell-derived erythroblasts arrested maturation and had severely impaired incorporation of iron-55 into heme.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dccf941b-8faf-5cca-b531-b3f08f30804a","slug":"mouse-slc25a37","display_name":"Mouse mitoferrin-1 / Slc25a37","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1007c432-b01d-5381-8756-60d6c68e072d","slug":"erythroid-heme-synthesis","display_name":"Erythroid heme synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/16511496.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cb2b06715c001133d1f1173d5084a2689170fa9a0e14f3b7b0a0cb5a7b6a5234\", \"start_char\": 0, \"end_char\": 1533, \"text_sha256\": \"cb2b06715c001133d1f1173d5084a2689170fa9a0e14f3b7b0a0cb5a7b6a5234\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Zebrafish mutant, mouse stem-cell knockout and cross-species rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mitoferrin loss and rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Species-specific loss/rescue evidence; no claim that plasma iron alone measures mitochondrial iron delivery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Zebrafish, mice and yeast","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Plenty of iron elsewhere would not replace this missing mitochondrial transport step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p16511496] Mitoferrin is essential for erythroid iron assimilation. (2006). https://pubmed.ncbi.nlm.nih.gov/16511496/ DOI: 10.1038/nature04512","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Developing erythroblasts and mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2e506fd2-be3d-5782-abbd-6383aff985f7","evidence_kind":"source_excerpt","locator":"Lines 472-483","start_line":472,"end_line":483,"excerpt":"### iron-mitoferrin-loss\nMfrn-null mouse embryonic-stem-cell-derived erythroblasts arrested maturation and had severely impaired incorporation of iron-55 into heme.\nCondition category: machinery_impairment\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Plenty of iron elsewhere would not replace this missing mitochondrial transport step.\norganism: Zebrafish, mice and yeast\ntissue_or_cell_type: Developing erythroblasts and mitochondria\nexperimental_model: Zebrafish mutant, mouse stem-cell knockout and cross-species rescue\nlimitations: Species-specific loss/rescue evidence; no claim that plasma iron alone measures mitochondrial iron delivery.\nexposure: Mitoferrin loss and rescue\nevidence_span: {\"source_cache\": \"artifacts/iron-research/16511496.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cb2b06715c001133d1f1173d5084a2689170fa9a0e14f3b7b0a0cb5a7b6a5234\", \"start_char\": 0, \"end_char\": 1533, \"text_sha256\": \"cb2b06715c001133d1f1173d5084a2689170fa9a0e14f3b7b0a0cb5a7b6a5234\"}\n[iron-p16511496] Mitoferrin is essential for erythroid iron assimilation. (2006). https://pubmed.ncbi.nlm.nih.gov/16511496/ DOI: 10.1038/nature04512","model_system":"Zebrafish mutant, mouse stem-cell knockout and cross-species rescue","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iron-p16511496] Mitoferrin is essential for erythroid iron assimilation. (2006). https://pubmed.ncbi.nlm.nih.gov/16511496/ DOI: 10.1038/nature04512","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1560864c-91d1-58dc-90c4-49bf61aba3de","stable_key":"import-e13b03e1-a614-543a-ac1b-97df18cfe30d","title":"Iron: absorption, trafficking, iron-dependent enzymes and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f9dab767a21c9bbfef3227707fe3c82953536bb1335599dd4ef93b5ea2dbf03f","revision_id":"77700d7f-2902-54b9-adae-8cbc77b24682","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}