{"id":"41e764c0-2d7c-5b80-8478-4176192b399c","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-ap1b-polarity","predicate":"supports","statement":"Depletion of the AP-1B mu1B subunit in polarized MDCK cells caused expressed human NIS to be missorted toward the apical membrane while retaining transport function.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ceb686bb-5a4a-5ff7-be26-988f7a32b604","mechanism_event_label":"Correct membrane placement depends on the cell’s sorting machinery.","subject":{"id":"d53440b4-da35-5445-9749-638a6a22631e","slug":"canine-ap1b","display_name":"Canine AP-1B clathrin adaptor complex","entity_type_key":"protein_complex"},"object":{"id":"7d463fdc-0698-59aa-9141-153e62c343a9","slug":"nis-basolateral-targeting","display_name":"NIS basolateral membrane targeting","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ceb686bb-5a4a-5ff7-be26-988f7a32b604","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-ap1b-polarity-event","event_type":"biochemical_relationship","label":"Correct membrane placement depends on the cell’s sorting machinery.","description":"Depletion of the AP-1B mu1B subunit in polarized MDCK cells caused expressed human NIS to be missorted toward the apical membrane while retaining transport function.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"role":"sorted_cargo","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d53440b4-da35-5445-9749-638a6a22631e","slug":"canine-ap1b","display_name":"Canine AP-1B clathrin adaptor complex","entity_type_key":"protein_complex"},"role":"sorting_complex","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7d463fdc-0698-59aa-9141-153e62c343a9","slug":"nis-basolateral-targeting","display_name":"NIS basolateral membrane targeting","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"AP-1B mu1B silencing compared with control MDCK-hNIS cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered epithelial model; apical uptake does not establish useful blood-to-thyroid delivery in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human NIS in canine MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Correct membrane placement depends on the cell’s sorting machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized epithelial plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c29c21ea-8583-5df5-ac0e-e273450a042f","evidence_kind":"source_excerpt","locator":"Lines 206-217","start_line":206,"end_line":217,"excerpt":"### iodine-trans-ap1b-polarity\nDepletion of the AP-1B mu1B subunit in polarized MDCK cells caused expressed human NIS to be missorted toward the apical membrane while retaining transport function.\nCondition category: machinery_impairment\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Correct membrane placement depends on the cell’s sorting machinery.\norganism: Human NIS in canine MDCK cells\ntissue_or_cell_type: Polarized epithelial plasma membrane\nexperimental_model: Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing\nlimitations: Engineered epithelial model; apical uptake does not establish useful blood-to-thyroid delivery in vivo.\nexposure: AP-1B mu1B silencing compared with control MDCK-hNIS cells.\ncross_nutrient: false\n[iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","model_system":"Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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