{"id":"41b7b10f-f09b-5e91-bcce-5d98b94ce655","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-mouse-ttp-pip2-binding","predicate":"binds","statement":"The deposited mouse alpha-TTP complex contains alpha-tocopherol and PI(4,5)P2, with phosphoinositide binding at the protein surface linked to opening of its ligand pocket.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ade3d6c1-0f36-5f59-ae2b-ad076606c470","mechanism_event_label":"A membrane phosphoinositide interacts with the vitamin E transfer protein.","subject":{"id":"90f49fd1-26fa-5d6f-a63d-46918d63f1e4","slug":"mouse-ttpa","display_name":"Mouse alpha-tocopherol transfer protein","entity_type_key":"protein"},"object":{"id":"af38e50f-cdfb-5d4b-a6fb-f742d1b23d41","slug":"phosphatidylinositol-4-5-bisphosphate","display_name":"Phosphatidylinositol 4,5-bisphosphate","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"ade3d6c1-0f36-5f59-ae2b-ad076606c470","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-mouse-ttp-pip2-binding-event","event_type":"biochemical_relationship","label":"A membrane phosphoinositide interacts with the vitamin E transfer protein.","description":"The deposited mouse alpha-TTP complex contains alpha-tocopherol and PI(4,5)P2, with phosphoinositide binding at the protein surface linked to opening of its ligand pocket.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"90f49fd1-26fa-5d6f-a63d-46918d63f1e4","slug":"mouse-ttpa","display_name":"Mouse alpha-tocopherol transfer protein","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"af38e50f-cdfb-5d4b-a6fb-f742d1b23d41","slug":"phosphatidylinositol-4-5-bisphosphate","display_name":"Phosphatidylinositol 4,5-bisphosphate","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"bound_cargo","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"X-ray complex PDB 3W68","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Crystallized protein with alpha-tocopherol and PI(4,5)P2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure is mouse protein; no dietary phosphorus requirement or human affinity value is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A membrane phosphoinositide interacts with the vitamin E transfer protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kono2013] Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency. (2013). https://pubmed.ncbi.nlm.nih.gov/23599266/ DOI: 10.1126/science.1233508","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein-lipid complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ba98d685-edf9-5416-9ce1-e220a4c64090","evidence_kind":"source_excerpt","locator":"Lines 324-335","start_line":324,"end_line":335,"excerpt":"### ve-transport-mouse-ttp-pip2-binding\nThe deposited mouse alpha-TTP complex contains alpha-tocopherol and PI(4,5)P2, with phosphoinositide binding at the protein surface linked to opening of its ligand pocket.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A membrane phosphoinositide interacts with the vitamin E transfer protein.\norganism: Mus musculus protein\ntissue_or_cell_type: Purified protein-lipid complex\nexperimental_model: X-ray complex PDB 3W68\nlimitations: Structure is mouse protein; no dietary phosphorus requirement or human affinity value is inferred.\nexposure: Crystallized protein with alpha-tocopherol and PI(4,5)P2.\ncross_nutrient: false\n[kono2013] Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency. (2013). https://pubmed.ncbi.nlm.nih.gov/23599266/ DOI: 10.1126/science.1233508","model_system":"X-ray complex PDB 3W68","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [kono2013] Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency. (2013). https://pubmed.ncbi.nlm.nih.gov/23599266/ DOI: 10.1126/science.1233508","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a90b6565e092c6a2da07f74cf758792c2cf63b40746260c8ff12bd64322c36f4","revision_id":"956793d1-23e4-5397-9291-25c1732a8e02","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}