{"id":"4141ab0f-57ec-5561-9078-ecccc62cc3a7","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-gluglu-receptor","predicate":"attenuates_sucrose_response","statement":"Coapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2f9e7315-0495-5597-86cf-409a383b2660","mechanism_event_label":"Coapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.","subject":{"id":"0cfbbc63-9b80-5c27-84ee-fa3e75896c05","slug":"glutamyl-glutamate","display_name":"L-Glutamyl-L-glutamate / Glu-Glu","entity_type_key":"peptide"},"object":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"2f9e7315-0495-5597-86cf-409a383b2660","stable_key":"c932089b-ebc9-5f39-b2e5-84af74f9f6d0:sucrose-gluglu-receptor-event","event_type":"observed_relationship","label":"Coapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.","description":"Coapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0cfbbc63-9b80-5c27-84ee-fa3e75896c05","slug":"glutamyl-glutamate","display_name":"L-Glutamyl-L-glutamate / Glu-Glu","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"82558bd0-1a84-574a-a04c-10609c9c5dba","slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Sucrose concentration series up to 150 mM; MSG or Glu-Glu/Glu-Asp concentration series; mutant imaging used 100 mM sucrose with 1 mM Glu-Glu or 50 mM MSG","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"120-second fluorescence acquisition; imaging at 30 seconds","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata inspected.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TAS1R2/TAS1R3 in Flp-In 293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Nutrient and peptide modulation of human sweet receptor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular response, not a direct binding assay or a demonstration that every food tastes less sweet. pH/osmolarity controls and agonist-specific responses limit interpretation. Doses differ among panels.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sucrose chapter; direct sucrose observations are distinguished from shared component metabolism.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sucrose","display_name":"Sucrose","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TAS1R2/TAS1R3 in Flp-In 293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Coapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Modulation of sweet taste by umami compounds via sweet taste receptor subunit hT1R2. (2015). https://pubmed.ncbi.nlm.nih.gov/25853419/ DOI: 10.1371/journal.pone.0124030","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro coapplication","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Sweet-receptor calcium signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"85d38b1d-40f1-5c67-a68f-2c005f6a735b","evidence_kind":"source_excerpt","locator":"Lines 91-101","start_line":91,"end_line":101,"excerpt":"## sucrose-gluglu-receptor\nCoapplied Glu-Glu attenuated the sucrose-evoked calcium response in cells expressing human TAS1R2/TAS1R3.\nModel/species: Human TAS1R2/TAS1R3 in Flp-In 293 cells\nTissue: Sweet-receptor calcium signaling\nExposure: Sucrose concentration series up to 150 mM; MSG or Glu-Glu/Glu-Asp concentration series; mutant imaging used 100 mM sucrose with 1 mM Glu-Glu or 50 mM MSG\nRoute: In vitro coapplication\nDuration: 120-second fluorescence acquisition; imaging at 30 seconds\nExposure scope: Nutrient and peptide modulation of human sweet receptor\nLimits: Cellular response, not a direct binding assay or a demonstration that every food tastes less sweet. pH/osmolarity controls and agonist-specific responses limit interpretation. Doses differ among panels.\nReference: Modulation of sweet taste by umami compounds via sweet taste receptor subunit hT1R2. (2015). https://pubmed.ncbi.nlm.nih.gov/25853419/ DOI: 10.1371/journal.pone.0124030\nAccess: Primary full-text methods/results and metadata inspected.","model_system":"Human TAS1R2/TAS1R3 in Flp-In 293 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8e24ddf4-84ee-57b4-9fd8-093547432ece","stable_key":"import-c932089b-ebc9-5f39-b2e5-84af74f9f6d0","title":"Sucrose: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific sucrose curation with shared canonical claims retained by identity. Primary-study citations, negative findings, exposure details and limitations preserved. Not publisher full text.","file_path":"","sha256":"5e68ccbade7a80fc03778495756f0eeeeba5038cd10d5bef8e33cb1e93e4b044","revision_id":"6edb8ffc-11c5-5cd9-852c-85adb71165a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}