{"id":"413c08ec-5fc4-5d99-8437-0cd76a3f0b29","stable_key":"1fb6218a-60d1-55ea-87fe-07d15d0fc46f:atorvastatin-reduces-myoblast-viability","predicate":"reduces","statement":"Atorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"575c47fe-e60b-5e09-9fdb-3b7a08f7ff54","mechanism_event_label":"Atorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.","subject":{"id":"64f4b2c7-afdf-5b9a-b6f9-015a7aa96eee","slug":"atorvastatin","display_name":"Atorvastatin","entity_type_key":"small_molecule"},"object":{"id":"1171c6b1-5e14-5a93-8e7c-ec115334c726","slug":"c2c12-myoblast-viability","display_name":"C2C12 myoblast viability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"575c47fe-e60b-5e09-9fdb-3b7a08f7ff54","stable_key":"1fb6218a-60d1-55ea-87fe-07d15d0fc46f:atorvastatin-reduces-myoblast-viability-event","event_type":"observed_relationship","label":"Atorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.","description":"Atorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64f4b2c7-afdf-5b9a-b6f9-015a7aa96eee","slug":"atorvastatin","display_name":"Atorvastatin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1171c6b1-5e14-5a93-8e7c-ec115334c726","slug":"c2c12-myoblast-viability","display_name":"C2C12 myoblast viability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7de977d-fed9-5b1c-8138-fd951442b067","slug":"simvastatin","display_name":"Simvastatin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Murine C2C12 myoblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Atorvastatin at IC50, alongside simvastatin and methyl-beta-cyclodextrin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"An IC50 exposure in a proliferating myoblast line is not a therapeutic muscle concentration in a person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Murine C2C12 myoblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Atorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Geranylgeraniol Prevents Statin-Dependent Myotoxicity in C2C12 Muscle Cells through RAP1 GTPase Prenylation and Cytoskeletal Stabilization. (2018). https://pubmed.ncbi.nlm.nih.gov/29951166/ DOI: 10.1155/2018/6463807","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Muscle cell viability and regeneration in vitro","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a7636d5c-8b40-5541-97ff-4ee281de6c7a","evidence_kind":"source_excerpt","locator":"Lines 111-120","start_line":111,"end_line":120,"excerpt":"## atorvastatin-reduces-myoblast-viability\nAtorvastatin at its half-maximal inhibitory concentration reduced the viability of proliferating murine C2C12 myoblasts.\nModel/species: Murine C2C12 myoblasts\nTissue/system: Muscle cell viability and regeneration in vitro\nExposure: Atorvastatin at IC50, alongside simvastatin and methyl-beta-cyclodextrin\nRoute: In vitro\nDuration: Not stated here\nLimits: An IC50 exposure in a proliferating myoblast line is not a therapeutic muscle concentration in a person.\nPrimary reference: Geranylgeraniol Prevents Statin-Dependent Myotoxicity in C2C12 Muscle Cells through RAP1 GTPase Prenylation and Cytoskeletal Stabilization. (2018). https://pubmed.ncbi.nlm.nih.gov/29951166/ DOI: 10.1155/2018/6463807\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4431d8c-34d7-5db7-9edf-a35f68feba39","stable_key":"import-1fb6218a-60d1-55ea-87fe-07d15d0fc46f","title":"Atorvastatin: mechanism of action from target occupancy to isoprenoids, transport, muscle and metabolism (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Findings obtained with mevastatin, simvastatin or the statin class are recorded against those subjects. Study-specific citations, doses, negative findings and limitations retained. 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