{"id":"4136faf5-1f29-5641-a8a4-967b38034eb2","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-repeated-phenylalanine","predicate":"increases_tested","statement":"Six adults consumed eight hourly 600 mg servings; phenylalanine rose modestly and plateaued after four to five servings within the reported normal postprandial range.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7e91f30c-c623-5d04-ab73-1b60c725c6dc","mechanism_event_label":"Repeated exposure did not produce unlimited accumulation in this protocol.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"53c53d5a-5af6-5901-ba09-bccf1f416178","slug":"human-plasma-phenylalanine","display_name":"Human plasma phenylalanine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e91f30c-c623-5d04-ab73-1b60c725c6dc","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-repeated-phenylalanine-event","event_type":"observed_relationship","label":"Repeated exposure did not produce unlimited accumulation in this protocol.","description":"Six adults consumed eight hourly 600 mg servings; phenylalanine rose modestly and plateaued after four to five servings within the reported normal postprandial range.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"53c53d5a-5af6-5901-ba09-bccf1f416178","slug":"human-plasma-phenylalanine","display_name":"Human plasma phenylalanine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human beverage crossover; 4.8 g total over eight servings.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small healthy-adult study; not a PKU population or a long-term trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Repeated exposure did not produce unlimited accumulation in this protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effect of repeated ingestion of aspartame-sweetened beverage on plasma amino acid, blood methanol, and blood formate concentrations in normal adults. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2566887/ · DOI 10.1016/0026-0495(89)90125-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"03ccaa99-033d-5148-ba63-418c5009fe6d","evidence_kind":"source_excerpt","locator":"Lines 106-112","start_line":106,"end_line":112,"excerpt":"## aspartame-repeated-phenylalanine\nRepeated exposure did not produce unlimited accumulation in this protocol.\nSix adults consumed eight hourly 600 mg servings; phenylalanine rose modestly and plateaued after four to five servings within the reported normal postprandial range.\nModel: Human beverage crossover; 4.8 g total over eight servings.\nLimitations: Small healthy-adult study; not a PKU population or a long-term trial.\nEvidence access: Primary abstract\nEffect of repeated ingestion of aspartame-sweetened beverage on plasma amino acid, blood methanol, and blood formate concentrations in normal adults. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2566887/ · DOI 10.1016/0026-0495(89)90125-x","model_system":"Human beverage crossover; 4.8 g total over eight servings.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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