{"id":"40b55e52-26f1-538e-9231-d8abb75d7b35","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-intercalated-bk-flow-secretion","predicate":"required-for","statement":"Intercalated-cell BKalpha deletion abolished flow-stimulated K secretion in isolated mouse CCDs from both sexes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c1505a36-6154-5f4c-97e9-fc4ab9a86dc4","mechanism_event_label":"Flow-dependent potassium secretion needs BK channels in intercalated cells.","subject":{"id":"3774e390-948f-57ec-b2ee-052930a9ec6c","slug":"kcnma1","display_name":"Large-conductance calcium-activated potassium channel alpha subunit","entity_type_key":"protein"},"object":{"id":"00c10917-2733-5c41-ad97-d2b51417e6dd","slug":"renal-potassium-secretion","display_name":"Distal renal potassium secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c1505a36-6154-5f4c-97e9-fc4ab9a86dc4","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-intercalated-bk-flow-secretion-event","event_type":"biochemical_relationship","label":"Flow-dependent potassium secretion needs BK channels in intercalated cells.","description":"Intercalated-cell BKalpha deletion abolished flow-stimulated K secretion in isolated mouse CCDs from both sexes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3774e390-948f-57ec-b2ee-052930a9ec6c","slug":"kcnma1","display_name":"Large-conductance calcium-activated potassium channel alpha subunit","entity_type_key":"protein"},"role":"causal-subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"00c10917-2733-5c41-ad97-d2b51417e6dd","slug":"renal-potassium-secretion","display_name":"Distal renal potassium secretion","entity_type_key":"cellular_process"},"role":"measured-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"secreted-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"channel-activating-ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9465acdc-1e9d-5e77-a954-e07dd8945385","slug":"luminal-flow","display_name":"Renal tubular luminal flow","entity_type_key":"cellular_process"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"A calcium-activated K channel connects tubular flow to K elimination.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 5; sex context Figure 8.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-specific knockout; high-K adaptation; microperfusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chronic blood K elevation occurred only in males; whole-animal urinary outputs showed compensation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Flow-dependent potassium secretion needs BK channels in intercalated cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[carrisoza-2020-intercalated-bk] Intercalated cell BKalpha subunit is required for flow-induced K+ secretion (2020). https://insight.jci.org/articles/view/130553 DOI: 10.1172/jci.insight.130553","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CCD intercalated cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"525f907f-cdad-537e-980c-dd1e5eada3d3","evidence_kind":"source_excerpt","locator":"Lines 356-367","start_line":356,"end_line":367,"excerpt":"### renal-intercalated-bk-flow-secretion\nIntercalated-cell BKalpha deletion abolished flow-stimulated K secretion in isolated mouse CCDs from both sexes.\nCondition category: machinery_impairment\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Flow-dependent potassium secretion needs BK channels in intercalated cells.\norganism: Mus musculus\ntissue_or_cell_type: CCD intercalated cells\nexperimental_model: Cell-specific knockout; high-K adaptation; microperfusion\nlimitations: Chronic blood K elevation occurred only in males; whole-animal urinary outputs showed compensation.\ncross_nutrient: A calcium-activated K channel connects tubular flow to K elimination.\nevidence_location: Figure 5; sex context Figure 8.\n[carrisoza-2020-intercalated-bk] Intercalated cell BKalpha subunit is required for flow-induced K+ secretion (2020). https://insight.jci.org/articles/view/130553 DOI: 10.1172/jci.insight.130553","model_system":"Cell-specific knockout; high-K adaptation; microperfusion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [carrisoza-2020-intercalated-bk] Intercalated cell BKalpha subunit is required for flow-induced K+ secretion (2020). https://insight.jci.org/articles/view/130553 DOI: 10.1172/jci.insight.130553","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}