{"id":"409be6e1-d993-567b-a29f-70ca3dba47e9","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:valine-propionyl-coa-lipogenesis","predicate":"supplies-carbon-to","statement":"Carbon-13 valine supplied the lipogenic propionyl-CoA precursor in cultured 3T3-L1 adipocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6a3dc0a6-8904-58ce-819c-492e991a0c05","mechanism_event_label":"Mouse fat-cell tracing placed valine carbon into the precursor that can enter B12-dependent metabolism or odd-chain fat synthesis.","subject":{"id":"ae40617e-3df7-5d48-8dd2-f16a0d3a8a25","slug":"valine","display_name":"L-Valine","entity_type_key":"small_molecule"},"object":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6a3dc0a6-8904-58ce-819c-492e991a0c05","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:valine-propionyl-coa-lipogenesis-event","event_type":"biochemical_relationship","label":"Mouse fat-cell tracing placed valine carbon into the precursor that can enter B12-dependent metabolism or odd-chain fat synthesis.","description":"Carbon-13 valine supplied the lipogenic propionyl-CoA precursor in cultured 3T3-L1 adipocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e90f1e9f-8b3d-52d9-a279-d6f6291dbbb8","slug":"odd-chain-fatty-acid-synthesis","display_name":"Odd-chain fatty acid synthesis","entity_type_key":"cellular_process"},"role":"downstream-route","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae40617e-3df7-5d48-8dd2-f16a0d3a8a25","slug":"valine","display_name":"L-Valine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text Results; Figures 6-8 and isotope model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Differentiated mouse 3T3-L1 adipocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"24-hour [U-13C]valine tracing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mouse cell culture; isotope labeling and model assumptions do not establish human dietary carbon fractions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mouse fat-cell tracing placed valine carbon into the precursor that can enter B12-dependent metabolism or odd-chain fat synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[crown-2015-bcaa-fatty-acids] Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes. (2015). https://pubmed.ncbi.nlm.nih.gov/26710334/ DOI: 10.1371/journal.pone.0145850","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adipocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"87831744-f97c-5aaf-867a-ff962e2ccd30","evidence_kind":"source_excerpt","locator":"Lines 1216-1228","start_line":1216,"end_line":1228,"excerpt":"### valine-propionyl-coa-lipogenesis\nCarbon-13 valine supplied the lipogenic propionyl-CoA precursor in cultured 3T3-L1 adipocytes.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mouse fat-cell tracing placed valine carbon into the precursor that can enter B12-dependent metabolism or odd-chain fat synthesis.\norganism: Mus musculus\ntissue_or_cell_type: Adipocytes\nexperimental_model: Differentiated mouse 3T3-L1 adipocytes\nlimitations: Mouse cell culture; isotope labeling and model assumptions do not establish human dietary carbon fractions.\nexposure: 24-hour [U-13C]valine tracing\ncross_nutrient: true\nevidence_location: Full text Results; Figures 6-8 and isotope model\n[crown-2015-bcaa-fatty-acids] Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes. 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