{"id":"40792db3-a78b-5962-ab40-5fc1c3ff68bf","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-synaptotagmin-mutant-sensitivity","predicate":"supports","statement":"A synaptotagmin-1 knock-in mutation reducing calcium affinity also reduces the calcium sensitivity of evoked neurotransmitter release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ab183d3d-f13e-5d25-a122-1dbb2970d5b1","mechanism_event_label":"Changing the calcium sensor changes the calcium needed to trigger release.","subject":{"id":"39c2a9ab-97b9-5a59-a016-e2e150e5c2b6","slug":"syt1","display_name":"Synaptotagmin 1 / SYT1","entity_type_key":"protein"},"object":{"id":"b1115a80-1f08-5f9d-a845-260667be514b","slug":"evoked-synaptic-vesicle-release","display_name":"Evoked synaptic vesicle release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ab183d3d-f13e-5d25-a122-1dbb2970d5b1","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-synaptotagmin-mutant-sensitivity-event","event_type":"loss_of_function","label":"Changing the calcium sensor changes the calcium needed to trigger release.","description":"A synaptotagmin-1 knock-in mutation reducing calcium affinity also reduces the calcium sensitivity of evoked neurotransmitter release.","status":"provisional","compartment":{"slug":"presynaptic-terminal","display_name":"Presynaptic terminal"},"participants":[{"entity":{"id":"39c2a9ab-97b9-5a59-a016-e2e150e5c2b6","slug":"syt1","display_name":"Synaptotagmin 1 / SYT1","entity_type_key":"protein"},"role":"impaired_sensor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"regulatory_ligand","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b1115a80-1f08-5f9d-a845-260667be514b","slug":"evoked-synaptic-vesicle-release","display_name":"Evoked synaptic vesicle release","entity_type_key":"cellular_process"},"role":"affected_process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2fa1d21d-5cd5-53e4-94f4-a74843d564fc","slug":"presynaptic-terminal","display_name":"Presynaptic terminal","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"compartment_description","value_text":"Presynaptic terminal","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse synaptotagmin-1 knock-in and cultured neuronal release measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Readily releasable pool size and spontaneous release were unchanged; this is a sensor mutation, not calcium deprivation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Changing the calcium sensor changes the calcium needed to trigger release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-fernandezchacon2001] Synaptotagmin I functions as a calcium regulator of release probability (2001). https://pubmed.ncbi.nlm.nih.gov/11242035/ DOI: 10.1038/35065004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"loss_of_function","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured neurons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [ca-fernandezchacon2001] Synaptotagmin I functions as a calcium regulator of release probability (2001). https://pubmed.ncbi.nlm.nih.gov/11242035/ DOI: 10.1038/35065004","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}