{"id":"4052fe18-76bd-5d9d-8742-3abcc60461df","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-iv-plasma","predicate":"changes","statement":"In a randomized pilot with eight NAD+ recipients and three saline controls, 750 mg NAD+ over six hours produced a significant plasma NAD increase at the six-hour endpoint, with no significant early rise during the first two hours.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"baf593b6-efb7-5512-ad88-760d74819daa","mechanism_event_label":"Infusion input and measured blood concentration need not rise together immediately.","subject":{"id":"35010247-8e1e-5ea0-ab54-f32b9956f825","slug":"human-intravenous-nad-intervention","display_name":"Intravenous NAD+ intervention in humans","entity_type_key":"cellular_process"},"object":{"id":"2107182b-07f5-5fea-8583-44889dbc7376","slug":"human-plasma-nad","display_name":"Human plasma NAD concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"baf593b6-efb7-5512-ad88-760d74819daa","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-iv-plasma-event","event_type":"observed_relationship","label":"Infusion input and measured blood concentration need not rise together immediately.","description":"In a randomized pilot with eight NAD+ recipients and three saline controls, 750 mg NAD+ over six hours produced a significant plasma NAD increase at the six-hour endpoint, with no significant early rise during the first two hours.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"35010247-8e1e-5ea0-ab54-f32b9956f825","slug":"human-intravenous-nad-intervention","display_name":"Intravenous NAD+ intervention in humans","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2107182b-07f5-5fea-8583-44889dbc7376","slug":"human-plasma-nad","display_name":"Human plasma NAD concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human pilot; approximately 3 micromoles/minute; serial plasma and urine sampling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small pharmacokinetic study; disappearance from plasma does not prove intact tissue uptake or clinical benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Infusion input and measured blood concentration need not rise together immediately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NAD. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31572171/ · DOI 10.3389/fnagi.2019.00257","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"0f41e4ae-e555-5b01-b8a0-9df8b44e5697","evidence_kind":"source_excerpt","locator":"Lines 284-290","start_line":284,"end_line":290,"excerpt":"## nad-plus-iv-plasma\nInfusion input and measured blood concentration need not rise together immediately.\nIn a randomized pilot with eight NAD+ recipients and three saline controls, 750 mg NAD+ over six hours produced a significant plasma NAD increase at the six-hour endpoint, with no significant early rise during the first two hours.\nModel: Human pilot; approximately 3 micromoles/minute; serial plasma and urine sampling.\nLimitations: Small pharmacokinetic study; disappearance from plasma does not prove intact tissue uptake or clinical benefit.\nEvidence access: Primary full text\nA Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NAD. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31572171/ · DOI 10.3389/fnagi.2019.00257","model_system":"Human pilot; approximately 3 micromoles/minute; serial plasma and urine sampling.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}