{"id":"40431c5e-f71b-5e35-a4ea-a5329f32140f","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-mtf1-mt1","predicate":"abolished","statement":"Mtf1-null mouse embryonic stem cells lacked detectable basal and zinc-induced Mt1 and Mt2 transcription.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cf586111-9d56-5c77-86b2-5fa111798b90","mechanism_event_label":"Without the transcription factor, the cell could not activate these zinc-binding proteins.","subject":{"id":"29f71faf-0c65-502e-a2e9-65b409ea8c56","slug":"mouse-mtf1-loss","display_name":"Mouse Mtf1 loss of function","entity_type_key":"cellular_process"},"object":{"id":"b96b70c5-85d2-5bca-8264-fa03cc392fcc","slug":"mouse-metallothionein-transcription","display_name":"Mouse metallothionein Mt1/Mt2 transcription","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cf586111-9d56-5c77-86b2-5fa111798b90","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-mtf1-mt1-event","event_type":"biochemical_relationship","label":"Without the transcription factor, the cell could not activate these zinc-binding proteins.","description":"Mtf1-null mouse embryonic stem cells lacked detectable basal and zinc-induced Mt1 and Mt2 transcription.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e83dc538-b8b1-5528-9807-277f5b646893","slug":"mouse-mtf1","display_name":"Mouse metal-regulatory transcription factor 1 / Mtf1","entity_type_key":"protein"},"role":"affected_machinery","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5776c608-7d4b-5276-b89c-e1199b23fa04","slug":"mouse-mt1","display_name":"Mouse metallothionein 1 / Mt1","entity_type_key":"protein"},"role":"regulated_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"31f62d6e-86b7-536d-91d3-19288bbec427","slug":"mouse-mt2","display_name":"Mouse metallothionein 2 / Mt2","entity_type_key":"protein"},"role":"regulated_protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"signal","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"29f71faf-0c65-502e-a2e9-65b409ea8c56","slug":"mouse-mtf1-loss","display_name":"Mouse Mtf1 loss of function","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b96b70c5-85d2-5bca-8264-fa03cc392fcc","slug":"mouse-metallothionein-transcription","display_name":"Mouse metallothionein Mt1/Mt2 transcription","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Mouse metal-regulatory transcription factor 1 / Mtf1 (affected_machinery); Mouse metallothionein 1 / Mt1 (regulated_protein); Mouse metallothionein 2 / Mt2 (regulated_protein); Zinc(II) ion (signal)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/8026472-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"6647e4132df7d568ef07c1a33501887c1682b18442af37dbac9f390cd212fea5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse embryonic stem cells lacking Mtf1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Mtf1-null versus control and restored-expression comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic transcription-factor loss is not nutritional zinc deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Without the transcription factor, the cell could not activate these zinc-binding proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-8026472] The transcription factor MTF-1 is essential for basal and heavy metal-induced metallothionein gene expression. (1994). https://pubmed.ncbi.nlm.nih.gov/8026472/ DOI: 10.1002/j.1460-2075.1994.tb06581.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic stem cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1dff9b99-376f-5489-80cf-436719f74469","evidence_kind":"source_excerpt","locator":"Lines 859-871","start_line":859,"end_line":871,"excerpt":"### zn-sig-mtf1-mt1\nMtf1-null mouse embryonic stem cells lacked detectable basal and zinc-induced Mt1 and Mt2 transcription.\nCondition category: machinery_impairment\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Without the transcription factor, the cell could not activate these zinc-binding proteins.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic stem cells\nexperimental_model: Mouse embryonic stem cells lacking Mtf1\nlimitations: Genetic transcription-factor loss is not nutritional zinc deficiency.\nexposure: Mtf1-null versus control and restored-expression comparisons.\ncross_nutrient: Mouse metal-regulatory transcription factor 1 / Mtf1 (affected_machinery); Mouse metallothionein 1 / Mt1 (regulated_protein); Mouse metallothionein 2 / Mt2 (regulated_protein); Zinc(II) ion (signal)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/8026472-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"6647e4132df7d568ef07c1a33501887c1682b18442af37dbac9f390cd212fea5\"}\n[zn-sig-8026472] The transcription factor MTF-1 is essential for basal and heavy metal-induced metallothionein gene expression. (1994). https://pubmed.ncbi.nlm.nih.gov/8026472/ DOI: 10.1002/j.1460-2075.1994.tb06581.x","model_system":"Mouse embryonic stem cells lacking Mtf1","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zn-sig-8026472] The transcription factor MTF-1 is essential for basal and heavy metal-induced metallothionein gene expression. (1994). https://pubmed.ncbi.nlm.nih.gov/8026472/ DOI: 10.1002/j.1460-2075.1994.tb06581.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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