{"id":"4030f00a-a1f5-5f7b-91bc-40b343c4f267","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-gja1-stability-null","predicate":"did_not_stabilize","statement":"Lycopene at 0.1 micromolar was inactive in the connexin-43 mRNA-stabilization assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e47f760b-8e12-506b-8a60-5da80c91adb6","mechanism_event_label":"Improved communication does not establish every proposed connexin mechanism.","subject":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"object":{"id":"0162eec2-33a5-5ccc-bfff-3c800f4aac7b","slug":"gja1-mrna-stability","display_name":"Human GJA1 / connexin-43 mRNA stability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e47f760b-8e12-506b-8a60-5da80c91adb6","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-gja1-stability-null-event","event_type":"biochemical_relationship","label":"Improved communication does not establish every proposed connexin mechanism.","description":"Lycopene at 0.1 micromolar was inactive in the connexin-43 mRNA-stabilization assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3a361e92-c843-5beb-bb20-21664c48ecc5","slug":"gja1","display_name":"Human connexin 43 / GJA1","entity_type_key":"protein"},"role":"communication_related_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0162eec2-33a5-5ccc-bfff-3c800f4aac7b","slug":"gja1-mrna-stability","display_name":"Human GJA1 / connexin-43 mRNA stability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/10620348.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\", \"start_char\": 0, \"end_char\": 1295, \"text_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gap-junction and promoter/mRNA assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lycopene 0.1 micromolar; acyclo-retinoic acid 1 or 50 micromolar depending on endpoint","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Communication, mRNA stability and RAR reporter responses are distinct; very high metabolite doses are not physiological proof.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human fetal skin fibroblasts and reporter systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Improved communication does not establish every proposed connexin mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p10620348] Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. (2000). https://pubmed.ncbi.nlm.nih.gov/10620348/ DOI: 10.1006/abbi.1999.1510","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Connexin-related intercellular communication","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"63fd5f98-515e-5a9f-b6f5-0380314205cd","evidence_kind":"source_excerpt","locator":"Lines 624-635","start_line":624,"end_line":635,"excerpt":"### lycopene-gja1-stability-null\nLycopene at 0.1 micromolar was inactive in the connexin-43 mRNA-stabilization assay.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Improved communication does not establish every proposed connexin mechanism.\norganism: Human fetal skin fibroblasts and reporter systems\ntissue_or_cell_type: Connexin-related intercellular communication\nexperimental_model: Gap-junction and promoter/mRNA assays\nlimitations: Communication, mRNA stability and RAR reporter responses are distinct; very high metabolite doses are not physiological proof.\nexposure: Lycopene 0.1 micromolar; acyclo-retinoic acid 1 or 50 micromolar depending on endpoint\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/10620348.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\", \"start_char\": 0, \"end_char\": 1295, \"text_sha256\": \"dfd11c7c19dcd07e092a601ff44e9c900b5ecd717d3661ac2aca48d5cac72011\"}\n[lycopene-p10620348] Stimulation of gap junctional communication: comparison of acyclo-retinoic acid and lycopene. 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