{"id":"4026a5c4-2b8f-5fdd-a50d-d62175c6a97c","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:glyphosate-shikimate","predicate":"increases_in_recorded_experiment","statement":"The 90-day glyphosate study recorded cecal shikimate accumulation consistent with pathway inhibition.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"354e59d0-8122-5270-beed-a41219c7bdf0","mechanism_event_label":"The 90-day glyphosate study recorded cecal shikimate accumulation consistent with pathway inhibition.","subject":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"object":{"id":"68a0690d-0447-5ad5-8e9d-2c3cd568d22e","slug":"rat-cecal-shikimate-accumulation","display_name":"Rat cecal shikimate accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"354e59d0-8122-5270-beed-a41219c7bdf0","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:glyphosate-shikimate-event","event_type":"experimental_observation","label":"The 90-day glyphosate study recorded cecal shikimate accumulation consistent with pathway inhibition.","description":"**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. 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[Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"68a0690d-0447-5ad5-8e9d-2c3cd568d22e","slug":"rat-cecal-shikimate-accumulation","display_name":"Rat cecal shikimate accumulation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"fe7a4dc3-4f06-586b-9457-2dd949e2fa36","slug":"shikimate","display_name":"Shikimate anion","entity_type_key":"small_molecule"},"role":"measured intermediate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. 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Pool accumulation is not increased downstream flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The 90-day glyphosate study recorded cecal shikimate accumulation consistent with pathway inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 37-37; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"326655cf-ebca-5f60-9e23-0084b76695df","evidence_kind":"source_excerpt","locator":"Lines 37-37","start_line":37,"end_line":37,"excerpt":"**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","model_system":"Female Sprague–Dawley rats; 0.5, 50 and 175 mg/kg/day glyphosate-equivalent groups; 12/group.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. 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