{"id":"401bbd68-80b6-5e9f-9b3a-1166d7dcc34b","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-citrin-calcium","predicate":"stimulates","statement":"Calcium on the external side of the inner mitochondrial membrane stimulated citrin-mediated exchange.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3fdcbaba-dcf2-5309-a57a-6e2c0985b756","mechanism_event_label":"Calcium is a regulatory signal for the transporter.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3fdcbaba-dcf2-5309-a57a-6e2c0985b756","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-citrin-calcium-event","event_type":"biochemical_relationship","label":"Calcium is a regulatory signal for the transporter.","description":"Calcium on the external side of the inner mitochondrial membrane stimulated citrin-mediated exchange.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"exchanged_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"exchanged_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5252ba8d-8e58-5fa5-abdb-7bc97ef85818","slug":"slc25a13","display_name":"Human citrin / SLC25A13","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/citrulline-research/11566871.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\", \"start_char\": 0, \"end_char\": 1249, \"text_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstituted transporter and transfected-cell assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Aspartate exchange for glutamate plus proton; external calcium stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell and liposome experiments; no evidence that calcium supplementation corrects citrin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Citrulline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human citrin and aralar proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Calcium is a regulatory signal for the transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[citrulline-p11566871] Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria. (2001). https://pubmed.ncbi.nlm.nih.gov/11566871/ DOI: 10.1093/emboj/20.18.5060","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Inner mitochondrial membrane transport; malate-aspartate shuttle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5fbf0851-9344-509a-9abd-64b7d7ceafe0","evidence_kind":"source_excerpt","locator":"Lines 372-383","start_line":372,"end_line":383,"excerpt":"### citrulline-citrin-calcium\nCalcium on the external side of the inner mitochondrial membrane stimulated citrin-mediated exchange.\nCondition category: normal\nnutrient_topic: Citrulline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Calcium is a regulatory signal for the transporter.\norganism: Human citrin and aralar proteins\ntissue_or_cell_type: Inner mitochondrial membrane transport; malate-aspartate shuttle\nexperimental_model: Reconstituted transporter and transfected-cell assays\nlimitations: Cell and liposome experiments; no evidence that calcium supplementation corrects citrin deficiency.\nexposure: Aspartate exchange for glutamate plus proton; external calcium stimulation\nevidence_span: {\"source_cache\": \"artifacts/citrulline-research/11566871.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\", \"start_char\": 0, \"end_char\": 1249, \"text_sha256\": \"25151a91c1f832fb2e3f86421ef070fb3a7c92390b5b1e7c0038e1db1d6a0734\"}\n[citrulline-p11566871] Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria. (2001). https://pubmed.ncbi.nlm.nih.gov/11566871/ DOI: 10.1093/emboj/20.18.5060","model_system":"Reconstituted transporter and transfected-cell assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [citrulline-p11566871] Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria. (2001). https://pubmed.ncbi.nlm.nih.gov/11566871/ DOI: 10.1093/emboj/20.18.5060","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"112fd375-aa2a-59d2-8afd-6b2849bb79ee","stable_key":"import-a9828b14-fbd7-57bf-9e01-0d52d1b42a1f","title":"Citrulline: arginine recycling, nitrogen disposal and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"3720442dbabc28cacefd2145c7c97bfb937df26dca8527fde219ecd3735ed558","revision_id":"41ac8493-b004-59d7-99b3-8a3ca7163d3a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}