{"id":"3ffb2ea4-2683-5ebd-95bf-d1240eb82782","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-alpha2b-binding","predicate":"binds","statement":"Agmatine competed at human alpha-2B adrenergic receptors expressed in transfected cells in the 1995 receptor-panel study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"68633e69-4a61-5ada-b8db-ebb491e052c2","mechanism_event_label":"An adrenergic receptor is another distinct interaction node.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"47dfde72-b3e4-514a-a478-c670b19bdaa2","slug":"adra2b","display_name":"Human alpha-2B adrenergic receptor / ADRA2B","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"68633e69-4a61-5ada-b8db-ebb491e052c2","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-alpha2b-binding-event","event_type":"observed_relationship","label":"An adrenergic receptor is another distinct interaction node.","description":"Agmatine competed at human alpha-2B adrenergic receptors expressed in transfected cells in the 1995 receptor-panel study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"47dfde72-b3e4-514a-a478-c670b19bdaa2","slug":"adra2b","display_name":"Human alpha-2B adrenergic receptor / ADRA2B","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human receptor expression and radioligand competition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The study establishes binding in its assay, not that agmatine reproduces every action of clonidine or norepinephrine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An adrenergic receptor is another distinct interaction node.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Comparison of the properties of agmatine and endogenous clonidine-displacing substance at imidazoline and alpha-2 adrenergic receptors. · 1995 · https://pubmed.ncbi.nlm.nih.gov/7853171/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3fe56bc7-86e2-5d32-b622-55d1a56f8793","evidence_kind":"source_excerpt","locator":"Lines 300-306","start_line":300,"end_line":306,"excerpt":"## agmatine-sulfate-alpha2b-binding\nAn adrenergic receptor is another distinct interaction node.\nAgmatine competed at human alpha-2B adrenergic receptors expressed in transfected cells in the 1995 receptor-panel study.\nModel: Human receptor expression and radioligand competition.\nLimitations: The study establishes binding in its assay, not that agmatine reproduces every action of clonidine or norepinephrine.\nEvidence access: Primary abstract\nComparison of the properties of agmatine and endogenous clonidine-displacing substance at imidazoline and alpha-2 adrenergic receptors. · 1995 · https://pubmed.ncbi.nlm.nih.gov/7853171/","model_system":"Human receptor expression and radioligand competition.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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