{"id":"3f7df2d9-5c5d-5543-a831-92137aed0442","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-fasting-knockdown","predicate":"knockdown_reduces","statement":"Three ODC1-directed siRNAs depleted polyamines and reduced starvation-induced autophagic flux.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6462b0c6-3c6c-5e5f-8c3c-feb91a982909","mechanism_event_label":"Genetic evidence complements the inhibitor experiment.","subject":{"id":"f8b362af-6bb4-5955-92a8-22ade1d2a716","slug":"odc1","display_name":"Human ornithine decarboxylase / ODC1","entity_type_key":"protein"},"object":{"id":"4a6dfde4-66ab-53ea-b528-f14961c6a2dd","slug":"human-cell-starvation-autophagic-flux","display_name":"Human U2OS/H4 starvation-induced autophagic flux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6462b0c6-3c6c-5e5f-8c3c-feb91a982909","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-fasting-knockdown-event","event_type":"observed_relationship","label":"Genetic evidence complements the inhibitor experiment.","description":"Three ODC1-directed siRNAs depleted polyamines and reduced starvation-induced autophagic flux.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f8b362af-6bb4-5955-92a8-22ade1d2a716","slug":"odc1","display_name":"Human ornithine decarboxylase / ODC1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4a6dfde4-66ab-53ea-b528-f14961c6a2dd","slug":"human-cell-starvation-autophagic-flux","display_name":"Human U2OS/H4 starvation-induced autophagic flux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human U2OS cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ODC1 supplies several polyamines; depletion is not spermidine-specific.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Genetic evidence complements the inhibitor experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Spermidine is essential for fasting-mediated autophagy and longevity. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39117797/ · DOI 10.1038/s41556-024-01468-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"266654cf-6131-5a45-bd7a-5af51e79174e","evidence_kind":"source_excerpt","locator":"Lines 198-204","start_line":198,"end_line":204,"excerpt":"## spermidine-fasting-knockdown\nGenetic evidence complements the inhibitor experiment.\nThree ODC1-directed siRNAs depleted polyamines and reduced starvation-induced autophagic flux.\nModel: Human U2OS cells.\nLimitations: ODC1 supplies several polyamines; depletion is not spermidine-specific.\nEvidence access: Primary full text\nSpermidine is essential for fasting-mediated autophagy and longevity. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39117797/ · DOI 10.1038/s41556-024-01468-x","model_system":"Human U2OS cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}