{"id":"3f69b3d0-603b-51ab-9df5-29dfac111854","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-ppar-alpha-reporter","predicate":"activates","statement":"PPAR-alpha transactivation increased at 10–100 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c9ab9c2b-2944-5cd7-8adf-440ce61e4aa9","mechanism_event_label":"Receptor subtype changed the direction of the reporter response.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"b8bec756-1210-5c97-9ad0-ff8974822305","slug":"astaxanthin-ppar-alpha-reporter","display_name":"PPAR alpha reporter response in the astaxanthin study; construct species unresolved","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c9ab9c2b-2944-5cd7-8adf-440ce61e4aa9","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-ppar-alpha-reporter-event","event_type":"observed_relationship","label":"Receptor subtype changed the direction of the reporter response.","description":"PPAR-alpha transactivation increased at 10–100 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b8bec756-1210-5c97-9ad0-ff8974822305","slug":"astaxanthin-ppar-alpha-reporter","display_name":"PPAR alpha reporter response in the astaxanthin study; construct species unresolved","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, methods 2.3 and results 3.1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"CHO-K1 transfection assays; receptor-construct species not assigned from the main-text methods.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reporter and purified-domain results are distinct. These culture exposures do not establish oral receptor engagement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Receptor subtype changed the direction of the reporter response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"24c57e20-18b0-58de-a908-e33597da7cc3","evidence_kind":"source_excerpt","locator":"Lines 494-500","start_line":494,"end_line":500,"excerpt":"## astaxanthin-ppar-alpha-reporter\nReceptor subtype changed the direction of the reporter response.\nPPAR-alpha transactivation increased at 10–100 micromolar.\nModel: CHO-K1 transfection assays; receptor-construct species not assigned from the main-text methods.\nLimitations: Reporter and purified-domain results are distinct. These culture exposures do not establish oral receptor engagement.\nEvidence access: Primary full text, methods 2.3 and results 3.1\nThe natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","model_system":"CHO-K1 transfection assays; receptor-construct species not assigned from the main-text methods.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}