{"id":"3f09a0a5-9292-5391-ab60-b6edb589840b","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-rat-toxicity-not-b2-depletion","predicate":"depletion_not_supported_as_cause_of","statement":"The rat toxicity study found neither tissue-flavin changes nor overt riboflavin-deficiency signs supporting B2 depletion as the cause of boric-acid testicular toxicity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a4102de7-0a03-55a2-b767-378ef918c927","mechanism_event_label":"The researchers tested the B2-depletion explanation and did not find support for it.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"79f13e3f-f563-537a-bfc3-20fd40d94ae9","slug":"testicular-atrophy","display_name":"Testicular atrophy","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a4102de7-0a03-55a2-b767-378ef918c927","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-rat-toxicity-not-b2-depletion-event","event_type":"biochemical_relationship","label":"The researchers tested the B2-depletion explanation and did not find support for it.","description":"The rat toxicity study found neither tissue-flavin changes nor overt riboflavin-deficiency signs supporting B2 depletion as the cause of boric-acid testicular toxicity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"toxic_exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"79f13e3f-f563-537a-bfc3-20fd40d94ae9","slug":"testicular-atrophy","display_name":"Testicular atrophy","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/7889890.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"73e5c915a0c7a8fa107ff62480430e94c1cf5c40648f37e08d0266394b05b70a\", \"start_char\": 0, \"end_char\": 1729, \"text_sha256\": \"73e5c915a0c7a8fa107ff62480430e94c1cf5c40648f37e08d0266394b05b70a\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"High-dose rat toxicity experiments with supporting cell assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"High boric-acid exposure; testis boron approximately 1–2 mM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High-exposure animal toxicity does not specify a human dietary threshold. The indexed abstract is truncated; no unreported plasminogen-activator or cAMP result is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The researchers tested the B2-depletion explanation and did not find support for it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p7889890] Mechanism of the testicular toxicity of boric acid in rats: in vivo and in vitro studies. (1994). https://pubmed.ncbi.nlm.nih.gov/7889890/ DOI: 10.1289/ehp.94102s799","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Testis, brain, tissue flavins and isolated Leydig cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5a092e23-b627-5bbf-b51d-dfbf93c2a4c6","evidence_kind":"source_excerpt","locator":"Lines 1158-1169","start_line":1158,"end_line":1169,"excerpt":"### boron-rat-toxicity-not-b2-depletion\nThe rat toxicity study found neither tissue-flavin changes nor overt riboflavin-deficiency signs supporting B2 depletion as the cause of boric-acid testicular toxicity.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The researchers tested the B2-depletion explanation and did not find support for it.\norganism: Rat\ntissue_or_cell_type: Testis, brain, tissue flavins and isolated Leydig cells\nexperimental_model: High-dose rat toxicity experiments with supporting cell assays\nlimitations: High-exposure animal toxicity does not specify a human dietary threshold. The indexed abstract is truncated; no unreported plasminogen-activator or cAMP result is inferred.\nexposure: High boric-acid exposure; testis boron approximately 1–2 mM\nevidence_span: {\"source_cache\": \"artifacts/boron-research/7889890.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"73e5c915a0c7a8fa107ff62480430e94c1cf5c40648f37e08d0266394b05b70a\", \"start_char\": 0, \"end_char\": 1729, \"text_sha256\": \"73e5c915a0c7a8fa107ff62480430e94c1cf5c40648f37e08d0266394b05b70a\"}\n[boron-p7889890] Mechanism of the testicular toxicity of boric acid in rats: in vivo and in vitro studies. (1994). https://pubmed.ncbi.nlm.nih.gov/7889890/ DOI: 10.1289/ehp.94102s799","model_system":"High-dose rat toxicity experiments with supporting cell assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p7889890] Mechanism of the testicular toxicity of boric acid in rats: in vivo and in vitro studies. (1994). https://pubmed.ncbi.nlm.nih.gov/7889890/ DOI: 10.1289/ehp.94102s799","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0a32c1c5c70cfb4a378f6ace35cf5b75d90ed3065ada91b9f9f64015d603c8e3","revision_id":"38f316f2-4344-542e-a638-0ac5402f678d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}