{"id":"3efc95fa-0b60-5d3e-9333-1ac82dfd9552","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-samtor-binding","predicate":"binds","statement":"SAM bound human SAMTOR with an approximate dissociation constant of 7 micromolar and disrupted SAMTOR interaction with GATOR1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f8df1a56-b8c2-51f2-b146-ff17772d3a01","mechanism_event_label":"The cell senses an activated methionine product rather than only the free amino acid.","subject":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"object":{"id":"0aadc8c9-0495-5791-baff-c975275a21e3","slug":"samtor","display_name":"Human SAM sensor / SAMTOR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f8df1a56-b8c2-51f2-b146-ff17772d3a01","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-samtor-binding-event","event_type":"observed_relationship","label":"The cell senses an activated methionine product rather than only the free amino acid.","description":"SAM bound human SAMTOR with an approximate dissociation constant of 7 micromolar and disrupted SAMTOR interaction with GATOR1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0aadc8c9-0495-5791-baff-c975275a21e3","slug":"samtor","display_name":"Human SAM sensor / SAMTOR","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b4222f3d-f87f-5649-97b5-086af8bcf31b","slug":"gator1","display_name":"Human GATOR1 complex","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HEK293T and purified-protein binding/interactions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding affinity is not a plasma target or supplement dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The cell senses an activated methionine product rather than only the free amino acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29123071/ · DOI 10.1126/science.aao3265","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"91eab93c-7388-5444-8211-a6b14593e1ab","evidence_kind":"source_excerpt","locator":"Lines 228-234","start_line":228,"end_line":234,"excerpt":"## methionine-samtor-binding\nThe cell senses an activated methionine product rather than only the free amino acid.\nSAM bound human SAMTOR with an approximate dissociation constant of 7 micromolar and disrupted SAMTOR interaction with GATOR1.\nModel: Human HEK293T and purified-protein binding/interactions.\nLimitations: Binding affinity is not a plasma target or supplement dose.\nEvidence access: Primary full text\nSAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29123071/ · DOI 10.1126/science.aao3265","model_system":"Human HEK293T and purified-protein binding/interactions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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