{"id":"3ef5acad-01ff-5110-b5b4-df5517f5c7ae","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-chop-expression","predicate":"decreases_expression_of","statement":"Boric-acid treatment decreased the pro-apoptotic CHOP/GADD153 response in DU-145 cells despite increasing several other ER-stress-associated genes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6aa183ba-4f5a-5420-ae54-5055fa977962","mechanism_event_label":"The stress response was selective; a signal linked to cell death decreased.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"a279b09a-257e-5461-a1fe-04e1c515c675","slug":"ddit3","display_name":"Human CHOP / GADD153 / DDIT3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6aa183ba-4f5a-5420-ae54-5055fa977962","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-chop-expression-event","event_type":"biochemical_relationship","label":"The stress response was selective; a signal linked to cell death decreased.","description":"Boric-acid treatment decreased the pro-apoptotic CHOP/GADD153 response in DU-145 cells despite increasing several other ER-stress-associated genes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a279b09a-257e-5461-a1fe-04e1c515c675","slug":"ddit3","display_name":"Human CHOP / GADD153 / DDIT3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/27587023.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\", \"start_char\": 0, \"end_char\": 1886, \"text_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Time-course immunoblotting and ER-stress gene expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 µM boric acid; early time points from 30 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression changes do not establish direct molecular targets or clinical effects. Total eIF2α abundance is distinct from Ser51 phosphorylation. The study did not activate every ER-stress branch.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The stress response was selective; a signal linked to cell death decreased.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p27587023] Activation of the EIF2α/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake. (2017). https://pubmed.ncbi.nlm.nih.gov/27587023/ DOI: 10.1007/s12011-016-0824-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Prostate cancer cell culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b8258cc5-946a-5268-8a85-3013c077ecf1","evidence_kind":"source_excerpt","locator":"Lines 482-493","start_line":482,"end_line":493,"excerpt":"### boron-chop-expression\nBoric-acid treatment decreased the pro-apoptotic CHOP/GADD153 response in DU-145 cells despite increasing several other ER-stress-associated genes.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The stress response was selective; a signal linked to cell death decreased.\norganism: Human DU-145 cells\ntissue_or_cell_type: Prostate cancer cell culture\nexperimental_model: Time-course immunoblotting and ER-stress gene expression\nlimitations: Expression changes do not establish direct molecular targets or clinical effects. Total eIF2α abundance is distinct from Ser51 phosphorylation. The study did not activate every ER-stress branch.\nexposure: 10 µM boric acid; early time points from 30 minutes\nevidence_span: {\"source_cache\": \"artifacts/boron-research/27587023.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\", \"start_char\": 0, \"end_char\": 1886, \"text_sha256\": \"421c3ea56e2b1058bb780928a63bb648246560a0ba3a070d82dc3fdd37ff0725\"}\n[boron-p27587023] Activation of the EIF2α/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake. (2017). https://pubmed.ncbi.nlm.nih.gov/27587023/ DOI: 10.1007/s12011-016-0824-y","model_system":"Time-course immunoblotting and ER-stress gene expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p27587023] Activation of the EIF2α/ATF4 and ATF6 Pathways in DU-145 Cells by Boric Acid at the Concentration Reported in Men at the US Mean Boron Intake. (2017). https://pubmed.ncbi.nlm.nih.gov/27587023/ DOI: 10.1007/s12011-016-0824-y","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0a32c1c5c70cfb4a378f6ace35cf5b75d90ed3065ada91b9f9f64015d603c8e3","revision_id":"38f316f2-4344-542e-a638-0ac5402f678d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}