{"id":"3ef572d6-3c6a-5d10-8f00-a0d48a20ef4a","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-mouse-tyr-compartment","predicate":"when_deleted_increases","statement":"Tyr knockout in mouse B16F10 cells increased melanosomal tyrosine about twofold without changing whole-cell tyrosine, while pigment intermediates were lost.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"524ee36b-1297-5b9f-8b39-2e0a881c5e05","mechanism_event_label":"A local buildup can accompany loss of the product it normally supplies.","subject":{"id":"2ae28539-a388-5624-83d0-984f2c92ab41","slug":"mouse-tyr","display_name":"Mouse tyrosinase Tyr","entity_type_key":"protein"},"object":{"id":"4929252c-070f-5264-abf2-c3e74122dfdf","slug":"mouse-melanosomal-tyrosine","display_name":"Mouse melanosomal tyrosine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"524ee36b-1297-5b9f-8b39-2e0a881c5e05","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-mouse-tyr-compartment-event","event_type":"observed_relationship","label":"A local buildup can accompany loss of the product it normally supplies.","description":"Tyr knockout in mouse B16F10 cells increased melanosomal tyrosine about twofold without changing whole-cell tyrosine, while pigment intermediates were lost.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2ae28539-a388-5624-83d0-984f2c92ab41","slug":"mouse-tyr","display_name":"Mouse tyrosinase Tyr","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4929252c-070f-5264-abf2-c3e74122dfdf","slug":"mouse-melanosomal-tyrosine","display_name":"Mouse melanosomal tyrosine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text; Fig. 1d–e","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse B16F10 knockout; MelanoIP metabolomics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Whole-cell and organelle concentrations are different measurements; this is not dietary deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A local buildup can accompany loss of the product it normally supplies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"MFSD12 mediates the import of cysteine into melanosomes and lysosomes. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33208952/ · DOI 10.1038/s41586-020-2937-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e7caa723-af7c-552c-9f83-383bd7956f95","evidence_kind":"source_excerpt","locator":"Lines 196-202","start_line":196,"end_line":202,"excerpt":"## l-tyrosine-mouse-tyr-compartment\nA local buildup can accompany loss of the product it normally supplies.\nTyr knockout in mouse B16F10 cells increased melanosomal tyrosine about twofold without changing whole-cell tyrosine, while pigment intermediates were lost.\nModel: Mouse B16F10 knockout; MelanoIP metabolomics.\nLimitations: Whole-cell and organelle concentrations are different measurements; this is not dietary deficiency.\nEvidence access: Primary full text; Fig. 1d–e\nMFSD12 mediates the import of cysteine into melanosomes and lysosomes. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33208952/ · DOI 10.1038/s41586-020-2937-x","model_system":"Mouse B16F10 knockout; MelanoIP metabolomics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7777f4440a8bfb14a04ff73e73392ccc45d8ee89f7853ecbcc0027d6f563b8a0","revision_id":"e1206111-3a75-5809-b8e9-231ff809ff1e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}