{"id":"3ee23e2c-fd5c-558d-a0dd-c3cd8e9a594b","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-mapk-blockade","predicate":"reduces","statement":"Bromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7814e9fb-b2c1-54b1-ad8f-3650d7a469ff","mechanism_event_label":"Bromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.","subject":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"object":{"id":"ce09ecdd-2d56-51b8-8655-b280a91dc0e4","slug":"salmonella-induced-erk-jnk-activation","display_name":"Salmonella-induced ERK/JNK activation in Caco-2 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7814e9fb-b2c1-54b1-ad8f-3650d7a469ff","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-mapk-blockade-event","event_type":"observed_relationship","label":"Bromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.","description":"Bromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ce09ecdd-2d56-51b8-8655-b280a91dc0e4","slug":"salmonella-induced-erk-jnk-activation","display_name":"Salmonella-induced ERK/JNK activation in Caco-2 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8708f3a5-4319-552b-9a35-0c8563e4331d","slug":"salmonella-typhimurium","display_name":"Salmonella enterica serovar Typhimurium","entity_type_key":"organism"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"cc602857-e4e2-5fb7-9a9d-62a2e810fa48","slug":"human-erk1-erk2","display_name":"Human ERK1/ERK2 kinases","entity_type_key":"protein_set"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"1a0e3c28-7395-5dab-bda8-0d4913046874","slug":"human-jnk1-jnk2","display_name":"Human JNK1/JNK2 assay pool","entity_type_key":"protein_set"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Bromelain with Salmonella Typhimurium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute infection assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 intestinal epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Blocking these kinase signals did not prevent invasion or barrier-resistance loss in the same study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Bromelain chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"}},{"dimension":"organism","value_text":"Human Caco-2 intestinal epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Bromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. (2002). https://pubmed.ncbi.nlm.nih.gov/11748167/ DOI: 10.1128/IAI.70.1.86-95.2002","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"ERK1/2 and JNK phosphorylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"344efbb4-1437-56c8-b441-e46e7b82b1a6","evidence_kind":"source_excerpt","locator":"Lines 77-86","start_line":77,"end_line":86,"excerpt":"## bromelain-mapk-blockade\nBromelain dose-dependently blocked Salmonella Typhimurium-induced ERK1/2 and JNK activation in human Caco-2 cells.\nModel/species: Human Caco-2 intestinal epithelial cells\nTissue/system: ERK1/2 and JNK phosphorylation\nExposure: Bromelain with Salmonella Typhimurium\nRoute: In vitro\nDuration: Acute infection assay\nLimits: Blocking these kinase signals did not prevent invasion or barrier-resistance loss in the same study.\nPrimary reference: Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. (2002). https://pubmed.ncbi.nlm.nih.gov/11748167/ DOI: 10.1128/IAI.70.1.86-95.2002\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human Caco-2 intestinal epithelial cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9d363b4e-f438-5664-9d74-99c2e359391d","stable_key":"import-773f772e-bf8e-5e52-a2a2-6dd694d67f5d","title":"Bromelain: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"b328dbb8082bea612bb2c40d6ff41fa670f6e62f714bd014c90627fcab86e95b","revision_id":"1c10d22e-ecdf-56ef-b820-bb21182a9577","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}