{"id":"3e898037-3d55-52c4-9f60-b770d5207401","stable_key":"9ee3c90a-c675-5dce-a105-4dc6909dfeab:ml349-endogenous-mavs-null","predicate":"has_contextual_experimental_result","statement":"Treatment with ML349 did not produce a reported increase in endogenous MAVS S-acylation in HEK293T.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"fcb3f6d2-969b-502f-b5a8-d1ff36871aae","mechanism_event_label":"ml349-endogenous-mavs-null","subject":{"id":"a160f6fb-092c-5df1-927c-4354e3af03f2","slug":"ml349","display_name":"ML349, experimental APT2 inhibitor","entity_type_key":"small_molecule"},"object":{"id":"5409e1d0-1a7e-5e16-bff9-6393de638035","slug":"human-mavs-s-acylation","display_name":"Endogenous MAVS S-acylation in human cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fcb3f6d2-969b-502f-b5a8-d1ff36871aae","stable_key":"9ee3c90a-c675-5dce-a105-4dc6909dfeab:ml349-endogenous-mavs-null","event_type":"experimental_result","label":"ml349-endogenous-mavs-null","description":"Treatment with ML349 did not produce a reported increase in endogenous MAVS S-acylation in HEK293T.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a160f6fb-092c-5df1-927c-4354e3af03f2","slug":"ml349","display_name":"ML349, experimental APT2 inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5409e1d0-1a7e-5e16-bff9-6393de638035","slug":"human-mavs-s-acylation","display_name":"Endogenous MAVS S-acylation in human cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2dd9d35-5ffc-5743-8681-5a5f0d0d0279","slug":"human-mavs","display_name":"Human mitochondrial antiviral-signaling protein / MAVS","entity_type_key":"protein"},"role":"measured endogenous protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human HEK293T; endogenous MAVS; acyl-biotin exchange with hydroxylamine-minus controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Inhibitor treatment; dose and duration unresolved; no RLR stimulation specified in S2C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Null under the reported assay, not proof of no effect at other sites, durations or activation states. Figure S2C and its legend visually reviewed. Exact inhibitor concentrations/durations were not located in the main methods or S2 legend; no matched exposure comparison with the Molecular Cell 2024 study is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"SI Appendix Figure S2C; main Results: MAVS Is S-Palmitoylated by ZDHHC7.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1073/pnas.2403392121","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d4131437-4c01-5c8e-ba52-1902dc063252","evidence_kind":"source_excerpt","locator":"Lines 6-12","start_line":6,"end_line":12,"excerpt":"Treatment with ML349 did not produce a reported increase in endogenous MAVS S-acylation in HEK293T.\nprimary_references: https://doi.org/10.1073/pnas.2403392121\nprimary_locator: SI Appendix Figure S2C; main Results: MAVS Is S-Palmitoylated by ZDHHC7.\norganism: Human\nexperimental_model: Human HEK293T; endogenous MAVS; acyl-biotin exchange with hydroxylamine-minus controls\nexposure: Inhibitor treatment; dose and duration unresolved; no RLR stimulation specified in S2C.\nlimitations: Null under the reported assay, not proof of no effect at other sites, durations or activation states. Figure S2C and its legend visually reviewed. Exact inhibitor concentrations/durations were not located in the main methods or S2 legend; no matched exposure comparison with the Molecular Cell 2024 study is established.","model_system":"Human HEK293T; endogenous MAVS; acyl-biotin exchange with hydroxylamine-minus controls","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of primary result and assay context.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dcc74980-d350-5911-b545-8c47ecdcf8fd","stable_key":"import-9ee3c90a-c675-5dce-a105-4dc6909dfeab","title":"MAVS inhibitor nulls and APT2-TBK1 immune feedback","document_type":"imported_text","citation_label":"Selected primary observations: 10.1073/pnas.2403392121; 10.1038/s41467-025-65081-8.","file_path":"","sha256":"16b3ee62ea6e0331133d1f7e9adbf7569a58fcbfda2b64c25a12f0dc4cdf4fe6","revision_id":"a5ea594c-6318-5060-b1c7-26e57336aa8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}