{"id":"3e740c74-f28f-54f5-957e-e164f6a30f45","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-podocyte-autophagy","predicate":"reduces","statement":"Fisetin reduced p70S6K phosphorylation, increased autophagosome formation and suppressed inflammasome readouts in mouse podocyte models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b00401f5-075b-5640-8c64-1fa697f1110b","mechanism_event_label":"Protein recycling and inflammatory signaling changed together.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"68a37da6-a40c-576c-817e-ab7775466825","slug":"mouse-podocyte-nlrp3-activation","display_name":"Mouse podocyte NLRP3 inflammasome activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b00401f5-075b-5640-8c64-1fa697f1110b","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-podocyte-autophagy-event","event_type":"observed_relationship","label":"Protein recycling and inflammatory signaling changed together.","description":"Fisetin reduced p70S6K phosphorylation, increased autophagosome formation and suppressed inflammasome readouts in mouse podocyte models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"68a37da6-a40c-576c-817e-ab7775466825","slug":"mouse-podocyte-nlrp3-activation","display_name":"Mouse podocyte NLRP3 inflammasome activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bffebe7d-585f-5975-bab6-49c744409f5e","slug":"mouse-cdkn1b","display_name":"Mouse p27 / Cdkn1b","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"763c166a-163a-5c5c-b136-2890efa8d822","slug":"mouse-rps6kb1","display_name":"Mouse S6 kinase 1 / Rps6kb1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse podocytes and diabetic kidney study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Autophagosome number alone does not establish complete autophagic flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Protein recycling and inflammatory signaling changed together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin Attenuates Diabetic Nephropathy-Induced Podocyte Injury by Inhibiting NLRP3 Inflammasome. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35126159/ · DOI 10.3389/fphar.2022.783706","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0961ce89-26d0-5939-a18f-a0af52b9e572","evidence_kind":"source_excerpt","locator":"Lines 400-406","start_line":400,"end_line":406,"excerpt":"## fisetin-podocyte-autophagy\nProtein recycling and inflammatory signaling changed together.\nFisetin reduced p70S6K phosphorylation, increased autophagosome formation and suppressed inflammasome readouts in mouse podocyte models.\nModel: Mouse podocytes and diabetic kidney study.\nLimitations: Autophagosome number alone does not establish complete autophagic flux.\nEvidence access: Primary abstract\nFisetin Attenuates Diabetic Nephropathy-Induced Podocyte Injury by Inhibiting NLRP3 Inflammasome. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35126159/ · DOI 10.3389/fphar.2022.783706","model_system":"Mouse podocytes and diabetic kidney study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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