{"id":"3dfd79f8-1504-54eb-8791-b66b57c46f54","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-comt-inhibition","predicate":"decreases_in_recorded_comparison","statement":"RA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9d02d054-8144-50d9-a816-8ed2f5628b9b","mechanism_event_label":"RA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"6b945a9f-6363-53b0-ac4f-5f0193752b21","slug":"comt-assay-preparation","display_name":"COMT preparation used in dietary-catechol methylation assay, organism not specified in abstract","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"9d02d054-8144-50d9-a816-8ed2f5628b9b","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-comt-inhibition-event","event_type":"experimental_observation","label":"RA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.","description":"### ra-comt-inhibition\n\nRA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.\nInhibition is distinct from RA methylation and from clinically meaningful endogenous-substrate competition.\nModel and exposure: Isolated RA from Blechnum brasiliense; enzyme species not specified in accessible abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | 2016 | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA concentration series","sequence_order":0,"notes":""},{"entity":{"id":"6b945a9f-6363-53b0-ac4f-5f0193752b21","slug":"comt-assay-preparation","display_name":"COMT preparation used in dietary-catechol methylation assay, organism not specified in abstract","entity_type_key":"protein_family"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"COMT methyl donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"COMT methyl-transfer product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA concentration series\", \"comparator\": \"Assay without RA\", \"endpoint\": \"RA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Isolated RA from Blechnum brasiliense; enzyme species not specified in accessible abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-comt-inhibition; Inhibition is distinct from RA methylation and from clinically meaningful endogenous-substrate competition.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"523b6e0c-cdcf-55fa-9ceb-0c15f22f167d","evidence_kind":"source_excerpt","locator":"Lines 113-119","start_line":113,"end_line":119,"excerpt":"### ra-comt-inhibition\n\nRA inhibited COMT in the 2016 assay with an IC50 of 26.7 micromolar.\nInhibition is distinct from RA methylation and from clinically meaningful endogenous-substrate competition.\nModel and exposure: Isolated RA from Blechnum brasiliense; enzyme species not specified in accessible abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Combining in vitro and in silico approaches to evaluate the multifunctional profile of rosmarinic acid from Blechnum brasiliense on targets related to neurodegeneration. | 2016 | https://pubmed.ncbi.nlm.nih.gov/27270453/ | https://doi.org/10.1016/j.cbi.2016.06.005 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Isolated RA from Blechnum brasiliense; enzyme species not specified in accessible abstract.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}