{"id":"3dc4ea0d-7bf7-5973-bc82-ac145bfb68d0","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-cftr-current","predicate":"inhibits","statement":"Steviol acutely reduced forskolin-stimulated apical chloride current in canine MDCK epithelia.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d94d859a-6ceb-5f85-8f59-f4dd5fb3fd2c","mechanism_event_label":"Chloride movement can determine fluid accumulation in a tissue model.","subject":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"object":{"id":"2b04e6b2-6fd0-5a0d-8f08-565c7f1e61cc","slug":"dog-cftr","display_name":"Canine cystic fibrosis transmembrane conductance regulator / CFTR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d94d859a-6ceb-5f85-8f59-f4dd5fb3fd2c","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-cftr-current-event","event_type":"observed_relationship","label":"Chloride movement can determine fluid accumulation in a tissue model.","description":"Steviol acutely reduced forskolin-stimulated apical chloride current in canine MDCK epithelia.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b04e6b2-6fd0-5a0d-8f08-565c7f1e61cc","slug":"dog-cftr","display_name":"Canine cystic fibrosis transmembrane conductance regulator / CFTR","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Canine kidney epithelial Ussing-chamber measurements; concentration-dependent bath exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The source identifies CFTR-associated current; this is not a human dietary chloride-depletion mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Chloride movement can determine fluid accumulation in a tissue model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Steviol reduces MDCK Cyst formation and growth by inhibiting CFTR channel activity and promoting proteasome-mediated CFTR degradation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23536832/ · DOI 10.1371/journal.pone.0058871","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"115c2ffc-ee85-5abb-bc4b-dbd5e4a04b56","evidence_kind":"source_excerpt","locator":"Lines 298-304","start_line":298,"end_line":304,"excerpt":"## stevia-cftr-current\nChloride movement can determine fluid accumulation in a tissue model.\nSteviol acutely reduced forskolin-stimulated apical chloride current in canine MDCK epithelia.\nModel: Canine kidney epithelial Ussing-chamber measurements; concentration-dependent bath exposure.\nLimitations: The source identifies CFTR-associated current; this is not a human dietary chloride-depletion mechanism.\nEvidence access: Primary abstract\nSteviol reduces MDCK Cyst formation and growth by inhibiting CFTR channel activity and promoting proteasome-mediated CFTR degradation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23536832/ · DOI 10.1371/journal.pone.0058871","model_system":"Canine kidney epithelial Ussing-chamber measurements; concentration-dependent bath exposure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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