{"id":"3dbbff8d-e475-5765-8a41-9668000f65c2","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-insulin-diaphragm","predicate":"inhibits","statement":"Millimolar phlorizin inhibited glucose transport in isolated rat diaphragm with and without insulin and added a smaller inhibition of glycogen synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"309549c7-2f28-5086-baef-7ff14f13d716","mechanism_event_label":"Millimolar phlorizin inhibited glucose transport in isolated rat diaphragm with and without insulin and added a smaller inhibition of glycogen synthesis.","subject":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"object":{"id":"44b5966c-e4c0-5f0f-97a2-f0af6445ca9c","slug":"rat-diaphragm-glucose-transport","display_name":"Glucose transport in isolated rat diaphragm","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"309549c7-2f28-5086-baef-7ff14f13d716","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-insulin-diaphragm-event","event_type":"observed_relationship","label":"Millimolar phlorizin inhibited glucose transport in isolated rat diaphragm with and without insulin and added a smaller inhibition of glycogen synthesis.","description":"Millimolar phlorizin inhibited glucose transport in isolated rat diaphragm with and without insulin and added a smaller inhibition of glycogen synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44b5966c-e4c0-5f0f-97a2-f0af6445ca9c","slug":"rat-diaphragm-glucose-transport","display_name":"Glucose transport in isolated rat diaphragm","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Millimolar phlorizin with or without insulin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(1977). https://pubmed.ncbi.nlm.nih.gov/889936/ DOI: 10.1016/s0300-9084(77)80058-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Sugar transport, glycogen synthesis and nucleotide turnover","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f990dbff-48f4-51c2-82ca-0895019a87ca","evidence_kind":"source_excerpt","locator":"Lines 121-130","start_line":121,"end_line":130,"excerpt":"## phlorizin-insulin-diaphragm\nMillimolar phlorizin inhibited glucose transport in isolated rat diaphragm with and without insulin and added a smaller inhibition of glycogen synthesis.\nModel/species: Isolated surviving rat diaphragm\nTissue/system: Sugar transport, glycogen synthesis and nucleotide turnover\nExposure: Millimolar phlorizin with or without insulin\nRoute: Ex vivo\nDuration: Acute\nLimits: This high-concentration extra-renal effect does not represent selective SGLT2 inhibition or typical human exposure.\nPrimary reference: The combined action of insulin and phlorizin on transport and metabolism of sugars and nucleotide turnover in the isolated rat diaphragm. (1977). https://pubmed.ncbi.nlm.nih.gov/889936/ DOI: 10.1016/s0300-9084(77)80058-8\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Isolated surviving rat diaphragm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7d4bfcb9-1665-5686-8b8a-1ad17ad6a4d2","stable_key":"import-bf161ddf-8b82-5d65-b86d-0b41245aac61","title":"Phlorizin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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