{"id":"3d853de3-a9e7-5628-bfb6-5a248e55769a","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-protein-decysteinylation","predicate":"reactivates_cysteinylated","statement":"TRP14 removed cysteinyl mixed-disulfide modifications from human peroxiredoxin 2 and restored its peroxide-reducing activity in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"456d09a9-77f1-5d24-a62f-e9101306e002","mechanism_event_label":"A cysteine modification can temporarily obstruct another antioxidant enzyme.","subject":{"id":"8e8b774c-00bd-5f89-b450-e0e5c4a7fb77","slug":"txndc17","display_name":"Human thioredoxin-related protein 14 / TXNDC17","entity_type_key":"protein"},"object":{"id":"b1c63fa2-9594-5e5c-8520-54a820fcf428","slug":"prdx2","display_name":"Human peroxiredoxin 2 / PRDX2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"456d09a9-77f1-5d24-a62f-e9101306e002","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-protein-decysteinylation-event","event_type":"observed_relationship","label":"A cysteine modification can temporarily obstruct another antioxidant enzyme.","description":"TRP14 removed cysteinyl mixed-disulfide modifications from human peroxiredoxin 2 and restored its peroxide-reducing activity in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e8b774c-00bd-5f89-b450-e0e5c4a7fb77","slug":"txndc17","display_name":"Human thioredoxin-related protein 14 / TXNDC17","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b1c63fa2-9594-5e5c-8520-54a820fcf428","slug":"prdx2","display_name":"Human peroxiredoxin 2 / PRDX2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"64fac263-c4d5-51c8-beba-d061868b0ce2","slug":"txnrd1","display_name":"TXNRD1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human Prx2; cysteinylation and hydrogen-peroxide reduction assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TRP14 repairs this modification; it is not being assigned the ordinary catalytic peroxide-reduction cycle of thioredoxin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A cysteine modification can temporarily obstruct another antioxidant enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38811853/ · DOI 10.1038/s44318-024-00117-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"06d6b04f-7b5a-5d18-9538-214616ac37cd","evidence_kind":"source_excerpt","locator":"Lines 84-90","start_line":84,"end_line":90,"excerpt":"## l-cysteine-protein-decysteinylation\nA cysteine modification can temporarily obstruct another antioxidant enzyme.\nTRP14 removed cysteinyl mixed-disulfide modifications from human peroxiredoxin 2 and restored its peroxide-reducing activity in vitro.\nModel: Recombinant human Prx2; cysteinylation and hydrogen-peroxide reduction assays.\nLimitations: TRP14 repairs this modification; it is not being assigned the ordinary catalytic peroxide-reduction cycle of thioredoxin.\nEvidence access: Primary full text\nTRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38811853/ · DOI 10.1038/s44318-024-00117-1","model_system":"Recombinant human Prx2; cysteinylation and hydrogen-peroxide reduction assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f722669e54eab08ffe7289f9d79ddfc443014ed8c4bc5ba10ce2635470d22498","revision_id":"8ae25609-a031-5782-9c62-6cad8767ea46","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}