{"id":"3d26b424-5f91-5003-a741-aabdbfeb2446","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-explant-production-null","predicate":"production_was_preserved_in","statement":"Hypoxia and/or inflammation did not reduce citrulline production during the six-hour human explant experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"21337ae9-9138-5581-93fa-e2717c74c954","mechanism_event_label":"Low circulating citrulline during illness cannot automatically be attributed to shut-down intestinal production.","subject":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"object":{"id":"3f4b97d0-e67d-5eb8-ab60-fb943ac8ec63","slug":"intestinal-citrulline-release","display_name":"Intestinal citrulline release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"21337ae9-9138-5581-93fa-e2717c74c954","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-explant-production-null-event","event_type":"biochemical_relationship","label":"Low circulating citrulline during illness cannot automatically be attributed to shut-down intestinal production.","description":"Hypoxia and/or inflammation did not reduce citrulline production during the six-hour human explant experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f4b97d0-e67d-5eb8-ab60-fb943ac8ec63","slug":"intestinal-citrulline-release","display_name":"Intestinal citrulline release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/citrulline-research/34882863.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3971162e4b915e3dadb6408a84cf3558526ee837e3446dd61c831f076f3fd901\", \"start_char\": 0, \"end_char\": 1801, \"text_sha256\": \"3971162e4b915e3dadb6408a84cf3558526ee837e3446dd61c831f076f3fd901\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Six-hour human duodenal explant experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Control, hypoxia, inflammation and combined exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Short ex-vivo exposure cannot explain all low citrulline in prolonged critical illness; increased utilization remains a proposed explanation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Citrulline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human biopsies from 25 selected patients","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Low circulating citrulline during illness cannot automatically be attributed to shut-down intestinal production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[citrulline-p34882863] Regulation of citrulline synthesis in human enterocytes: Role of hypoxia and inflammation. (2022). https://pubmed.ncbi.nlm.nih.gov/34882863/ DOI: 10.1002/biof.1810","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal citrulline production","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5afc6ec2-2afd-5aed-9ae3-0752d8fc5565","evidence_kind":"source_excerpt","locator":"Lines 1022-1033","start_line":1022,"end_line":1033,"excerpt":"### citrulline-explant-production-null\nHypoxia and/or inflammation did not reduce citrulline production during the six-hour human explant experiment.\nCondition category: normal\nnutrient_topic: Citrulline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Low circulating citrulline during illness cannot automatically be attributed to shut-down intestinal production.\norganism: Human biopsies from 25 selected patients\ntissue_or_cell_type: Intestinal citrulline production\nexperimental_model: Six-hour human duodenal explant experiment\nlimitations: Short ex-vivo exposure cannot explain all low citrulline in prolonged critical illness; increased utilization remains a proposed explanation.\nexposure: Control, hypoxia, inflammation and combined exposure\nevidence_span: {\"source_cache\": \"artifacts/citrulline-research/34882863.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3971162e4b915e3dadb6408a84cf3558526ee837e3446dd61c831f076f3fd901\", \"start_char\": 0, \"end_char\": 1801, \"text_sha256\": \"3971162e4b915e3dadb6408a84cf3558526ee837e3446dd61c831f076f3fd901\"}\n[citrulline-p34882863] Regulation of citrulline synthesis in human enterocytes: Role of hypoxia and inflammation. 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