{"id":"3d0f6ed3-2184-508d-928b-3d5d689c7fdc","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:early-k-deprivation-aqp2-autophagic-localization","predicate":"deprivation-increases","statement":"After one day of potassium deprivation, AQP2 localized in autophagic compartments in rat inner medullary collecting-duct cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cb8a8f85-7d04-55d3-8f69-2b83a4eb9c55","mechanism_event_label":"Water-channel disposal began early, alongside AQP2 loss.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"6dd3c661-377b-51df-a1cf-9d51cbcf5315","slug":"aqp2-autophagic-localization","display_name":"AQP2 localization in autophagic compartments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cb8a8f85-7d04-55d3-8f69-2b83a4eb9c55","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:early-k-deprivation-aqp2-autophagic-localization-event","event_type":"observed_intervention","label":"Water-channel disposal began early, alongside AQP2 loss.","description":"After one day of potassium deprivation, AQP2 localized in autophagic compartments in rat inner medullary collecting-duct cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6dd3c661-377b-51df-a1cf-9d51cbcf5315","slug":"aqp2-autophagic-localization","display_name":"AQP2 localization in autophagic compartments","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0cf1432e-2f5f-542c-8980-6894da46cce6","slug":"aqp2","display_name":"Aquaporin-2 / AQP2","entity_type_key":"protein"},"role":"localized-protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"affected-transport-substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"endpoint","value_text":"After one day of potassium deprivation, AQP2 localized in autophagic compartments in rat inner medullary collecting-duct cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Rats fed potassium-free diet for one day; IMCD proteomics, immunoblotting, immunogold electron microscopy, and refeeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats fed potassium-free diet for one day; IMCD proteomics, immunoblotting, immunogold electron microscopy, and refeeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Autophagy inhibition was not performed; colocalization and recovery do not establish that autophagic AQP2 degradation alone caused polyuria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Water-channel disposal began early, alongside AQP2 loss.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[khositseth-2015-aqp2] Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus (2015). https://www.nature.com/articles/srep18311 DOI: 10.1038/srep18311","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"inner medullary collecting duct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"61a2f53a-3228-5aa9-866a-eed58fc6a9e4","evidence_kind":"source_excerpt","locator":"Lines 1399-1410","start_line":1399,"end_line":1410,"excerpt":"### early-k-deprivation-aqp2-autophagic-localization\nAfter one day of potassium deprivation, AQP2 localized in autophagic compartments in rat inner medullary collecting-duct cells.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Water-channel disposal began early, alongside AQP2 loss.\norganism: Rattus norvegicus\ntissue_or_cell_type: inner medullary collecting duct\nexperimental_model: Rats fed potassium-free diet for one day; IMCD proteomics, immunoblotting, immunogold electron microscopy, and refeeding.\nlimitations: Autophagy inhibition was not performed; colocalization and recovery do not establish that autophagic AQP2 degradation alone caused polyuria.\nexperimental-exposure: Rats fed potassium-free diet for one day; IMCD proteomics, immunoblotting, immunogold electron microscopy, and refeeding.\nendpoint: After one day of potassium deprivation, AQP2 localized in autophagic compartments in rat inner medullary collecting-duct cells.\n[khositseth-2015-aqp2] Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus (2015). https://www.nature.com/articles/srep18311 DOI: 10.1038/srep18311","model_system":"Rats fed potassium-free diet for one day; IMCD proteomics, immunoblotting, immunogold electron microscopy, and refeeding.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [khositseth-2015-aqp2] Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus (2015). https://www.nature.com/articles/srep18311 DOI: 10.1038/srep18311","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}