{"id":"3c9338cc-ac96-503a-85df-5a1a90802ea1","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-dhrs3-activates-rdh10","predicate":"activates","statement":"DHRS3 coexpression enhanced RDH10 retinol dehydrogenase activity independently of DHRS3 catalytic activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2621eb70-6a4c-5d66-a8f5-ea82b893552c","mechanism_event_label":"The two enzymes mutually stimulate one another.","subject":{"id":"98441da8-56db-598c-9483-00e9c712656c","slug":"dhrs3","display_name":"Dehydrogenase/reductase 3 / DHRS3","entity_type_key":"protein"},"object":{"id":"89b4b04a-7637-56f4-ac55-501ad31d1f61","slug":"rdh10","display_name":"Retinol dehydrogenase 10 / RDH10","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2621eb70-6a4c-5d66-a8f5-ea82b893552c","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-dhrs3-activates-rdh10-event","event_type":"biochemical_relationship","label":"The two enzymes mutually stimulate one another.","description":"DHRS3 coexpression enhanced RDH10 retinol dehydrogenase activity independently of DHRS3 catalytic activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4d9421aa-82ac-5a6d-87c1-232e7d21c403","slug":"dhrs3-y188a","display_name":"DHRS3 Y188A","entity_type_key":"protein_state"},"role":"experimental-machinery-state","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"98441da8-56db-598c-9483-00e9c712656c","slug":"dhrs3","display_name":"Dehydrogenase/reductase 3 / DHRS3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89b4b04a-7637-56f4-ac55-501ad31d1f61","slug":"rdh10","display_name":"Retinol dehydrogenase 10 / RDH10","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Coexpression including inactive DHRS3 constructs.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Net retinoic-acid output depends on both opposing reactions and cellular conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens recombinant proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"outcome","value_text":"DHRS3 coexpression enhanced RDH10 retinol dehydrogenase activity independently of DHRS3 catalytic activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The two enzymes mutually stimulate one another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Sf9 microsomes and HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ae669b68-b8ff-516a-871f-f6b8cf930cbf","evidence_kind":"source_excerpt","locator":"Lines 545-557","start_line":545,"end_line":557,"excerpt":"### va-dhrs3-activates-rdh10\nDHRS3 coexpression enhanced RDH10 retinol dehydrogenase activity independently of DHRS3 catalytic activity.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The two enzymes mutually stimulate one another.\norganism: Homo sapiens recombinant proteins\ntissue_or_cell_type: Sf9 microsomes and HEK293 cells\nexperimental_model: Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.\nlimitations: Net retinoic-acid output depends on both opposing reactions and cellular conditions.\nexposure: Coexpression including inactive DHRS3 constructs.\noutcome: DHRS3 coexpression enhanced RDH10 retinol dehydrogenase activity independently of DHRS3 catalytic activity.\nevidence_location: Abstract\n[va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","model_system":"Human RDH10/DHRS3 coexpression in HEK293 and Sf9 cells, plus Dhrs3-null embryos.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-adams-2014] The retinaldehyde reductase activity of DHRS3 is reciprocally activated by retinol dehydrogenase 10 to control retinoid homeostasis (2014). https://pmc.ncbi.nlm.nih.gov/articles/PMC4031538/ DOI: 10.1074/jbc.M114.552257","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}