{"id":"3c92568d-baca-5ea5-a0a1-2eea49c12116","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-copper-oxidation","predicate":"suppresses","statement":"Ergothioneine at 0.1-1 mM protected DNA and albumin against 0.1 mM copper with ascorbate or peroxide.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0a9abb94-30a5-5c51-a494-b2caeae879dd","mechanism_event_label":"Binding the catalyst can protect several targets.","subject":{"id":"b9c1084d-e596-5f11-81b9-bf09dda3556e","slug":"ergothioneine-copper-i-complex","display_name":"Ergothioneine-copper(I) complex","entity_type_key":"small_molecule"},"object":{"id":"3c41c0c3-13d0-5a57-9406-16084dfcdf27","slug":"copper-driven-dna-protein-oxidation","display_name":"Copper-driven DNA and protein oxidation in chemical assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0a9abb94-30a5-5c51-a494-b2caeae879dd","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-copper-oxidation-event","event_type":"observed_relationship","label":"Binding the catalyst can protect several targets.","description":"Ergothioneine at 0.1-1 mM protected DNA and albumin against 0.1 mM copper with ascorbate or peroxide.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b9c1084d-e596-5f11-81b9-bf09dda3556e","slug":"ergothioneine-copper-i-complex","display_name":"Ergothioneine-copper(I) complex","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3c41c0c3-13d0-5a57-9406-16084dfcdf27","slug":"copper-driven-dna-protein-oxidation","display_name":"Copper-driven DNA and protein oxidation in chemical assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"162020b5-1fa5-526e-8405-452891245722","slug":"copper-i","display_name":"Copper(I) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free copper/ascorbate and copper/peroxide systems.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Exposure and free-metal speciation limit extrapolation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Binding the catalyst can protect several targets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ergothioneine prevents copper-induced oxidative damage to DNA and protein by forming a redox-inactive ergothioneine-copper complex. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21047085/ · DOI 10.1021/tx100214t","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"74708eb3-99d0-5634-af03-357d3d876190","evidence_kind":"source_excerpt","locator":"Lines 128-134","start_line":128,"end_line":134,"excerpt":"## ergothioneine-copper-oxidation\nBinding the catalyst can protect several targets.\nErgothioneine at 0.1-1 mM protected DNA and albumin against 0.1 mM copper with ascorbate or peroxide.\nModel: Cell-free copper/ascorbate and copper/peroxide systems.\nLimitations: Exposure and free-metal speciation limit extrapolation.\nEvidence access: Primary abstract\nErgothioneine prevents copper-induced oxidative damage to DNA and protein by forming a redox-inactive ergothioneine-copper complex. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21047085/ · DOI 10.1021/tx100214t","model_system":"Cell-free copper/ascorbate and copper/peroxide systems.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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